文章摘要
刘春花,赵长臣,罗霞,江小燕,巩华,黄志斌.黄芪多糖对草鱼非特异免疫功能的影响[J].上海海洋大学学报,2017,26(4):511-518
黄芪多糖对草鱼非特异免疫功能的影响
Effect of astragalus polysaccharides on nonspecific immunity of grass carp (Ctenopharyngodon idellus)
投稿时间:2016-12-16  修订日期:2017-05-08
DOI:10.12024/jsou.20161201921
中文关键词: 黄芪多糖  草鱼  非特异免疫功能
英文关键词: astragalus polysaccharides  grass carp (Ctenopharyngodon idellus)  nonspecific immunity
基金项目:中国水产科学研究院基本科研业务费项目(2016HY-ZD0502);广东省科技计划项目(2016B020234003,2016B090920089)
作者单位E-mail
刘春花 中国水产科学研究院珠江水产研究所, 广东 广州 510380;广州普麟生物制品有限公司, 广东 广州 510380  
赵长臣 中国水产科学研究院珠江水产研究所, 广东 广州 510380;广州普麟生物制品有限公司, 广东 广州 510380  
罗霞 中国水产科学研究院珠江水产研究所, 广东 广州 510380;广州普麟生物制品有限公司, 广东 广州 510380  
江小燕 中国水产科学研究院珠江水产研究所, 广东 广州 510380;广州普麟生物制品有限公司, 广东 广州 510380  
巩华 中国水产科学研究院珠江水产研究所, 广东 广州 510380  
黄志斌 中国水产科学研究院珠江水产研究所, 广东 广州 510380 hzb1393@163.com 
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中文摘要:
      旨在探讨饲料中添加不同水平的黄芪多糖(Astragalus polysaccharides,APS)对草鱼(Ctenopharyngodon idellus)非特异性免疫指标的影响。将600尾健康草鱼随机分成Ⅰ-Ⅳ组,在基础饲料中分别添加黄芪多糖0、10.0、30.0、50.0 mg/(kg·d),于D0、D8、D15、D22、D29测定草鱼白细胞的吞噬百分比(PP)、吞噬指数(PI)及血清总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)与溶菌酶(LSZ)活力等指标,D29时对4组草鱼进行嗜水气单胞菌(Aeromonas hydrophila)攻毒实验。结果表明:与对照组相比,饲料中添加实验剂量的黄芪多糖可不同程度提高供试草鱼血液白细胞的吞噬百分比和吞噬指数,各实验组草鱼血清中T-SOD、CAT、LSZ活性均呈增加趋势,投喂实验剂量的黄芪多糖均可显著降低嗜水气单胞菌攻毒后草鱼的死亡率(P<0.05)。研究表明,在饲料中添加适宜水平的黄芪多糖可提高草鱼的非特异性免疫功能和对嗜水气单胞菌感染的抵抗力,每千克鱼体质量的推荐剂量为10~30 mg/d。
英文摘要:
      This experiment was conducted to investigate the effect of astragalus polysaccharides (APS) on nonspecific immunity of grass carp (Ctenopharyngodon idellus). 600 healthy grass carps were randomly divided into Group Ⅰ-Ⅳ, fed with the basal diet added with 0, 10.0, 30.0, 50.0 mg APS per kilogram body weight everyday respectively. Phagocytic percentage (PP), phagocytic index (PI) of leukocytes in blood and the activities of total superoxide dismutase (T-SOD), catalase (CAT) and lysozyme (LSZ) in serum were determined on D0, D8, D15, D22 and D29. On the 29th day of feeding, 20 grass carps of each group were infected by Aeromonas hydrophila and mortalities were recorded. The results indicated that APS added in diets can increase PP and PI of leukocytes, and enhance the activities of serum T-SOD, CAT, LSZ. The mortality rate of grass carps infected by A. hydrophila was significantly decreased when fed by APS (P<0.05) and the protective rate of Group Ⅱ-Ⅳ were 54.3%±6.4%, 52.9%±12.5% and 44.2%±4.8% respectively. It suggested that APS can enhance nonspecific immunity function of grass carp, and the effect of low dose was superior to high dose. The recommended dose of APS added to the diet applying in aquaculture was 10-30 mg/d per kilogram body weight.
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