文章摘要
刘丽,姜晓东,吴旭干,邓登,成永旭.育肥饲料中植物油替代鱼油对中华绒螯蟹生殖性能的影响[J].上海海洋大学学报,2017,26(4):501-510
育肥饲料中植物油替代鱼油对中华绒螯蟹生殖性能的影响
Effects of dietary fish oil replacement by vegetable oils on reproductive performance of Chinese mitten crab (Eriocheir sinensis)
投稿时间:2017-02-25  修订日期:2017-03-20
DOI:10.12024/jsou.20170201962
中文关键词: 中华绒螯蟹  育肥饲料  植物油  鱼油  生殖性能  脂肪酸组成
英文关键词: Eriocheir sinensis  fattening diet  vegetable oil  fish oil  proximate composition  fatty acid composition
基金项目:国家自然科学基金面上项目(31572630,31472287);科技部港澳台科技合作专项(2014DFT30270);上海市科技兴农推广项目〔沪农科推字(2015)第1-7号〕;上海市科学技术委员会科研项目(13320502100);上海高校水产学一流学科建设项目(2015-62-0908)
作者单位E-mail
刘丽 上海海洋大学 农业部淡水种质资源重点试验室, 上海 201306  
姜晓东 上海海洋大学 农业部淡水种质资源重点试验室, 上海 201306  
吴旭干 上海海洋大学 农业部淡水种质资源重点试验室, 上海 201306 xgwu@shou.edu.cn 
邓登 深圳市澳华农牧有限公司, 广东 深圳 518054  
成永旭 上海海洋大学 农业部淡水种质资源重点试验室, 上海 201306;上海海洋大学 上海市教委水产动物遗传育种协同创新中心, 上海 201306  
摘要点击次数: 241
全文下载次数: 254
中文摘要:
      采用豆油和菜籽油混合(1∶1)替代鱼油,研究了育肥饲料中不同鱼油替代水平对中华绒螯蟹(Eriocheir sinensis,简称河蟹)生殖性能、卵径、单卵质量和胚胎生化组成的影响。实验分为5组,饲料1#~5#鱼油替代水平分别为0、25%、50%、75%和100%。每个饲料组设置3个重复,每个重复放入雌蟹和雄蟹各25只,养殖60 d后进行交配试验,比较其生殖性能和胚胎生化组成。结果表明:(1)饲料3#组雌蟹在交配后第10天开始抱卵,其余组均在第15天开始抱卵,饲料1#、3#和5#组的最终抱卵率均为100%,而2#与4#组抱卵率为80%左右;饲料4#的产卵量、生殖力和生殖指数略高于其他各组,但各组间均无显著差异(P > 0.05)。(2)就胚胎质量而言,饲料1#组的卵径相对较大,但各饲料组的卵径、单卵湿重和干重的差异均不显著(P > 0.05)。(3)各饲料组胚胎中的水分、粗蛋白、粗脂肪和脂肪酸组成均无显著差异(P > 0.05),22∶6n3含量呈下降趋势,18∶2n6和∑n-6PUFA/∑n-3PUFA 呈上升趋势。因此,育肥饲料中采用豆油和菜籽油混合替代鱼油,投喂性腺发育中期的河蟹,对其生殖性能、胚胎质量及生化组成均没有显著影响,育肥饲料75%左右的鱼油替代水平可以适当提高其产卵量和生殖力。
英文摘要:
      This study was conducted to investigate the effects of different fish oil replacement levels on reproductive performance, egg diameter, individual egg wet weight and dry weight, proximate composition and fatty acid composition in the eggs produced by Chinese mitten crab (Eriocheir sinensis). Five isonitrogenous and isolipidic fattening diets were formulated by the blending vegetable oils(soybean oil: rapeseed oil = 1:1) to replace 0, 25%, 50%, 75% and 100% of fish oil(defined as feed 1#, 2#, 3#, 4# and 5#,respectively).Each experimental diet was fed to three replicates and each replicate consisted of 25 females and 25 males.After 60-day feeding, four females and two males from each replicate were randomly transferred into the brackish water tanks for the mating, and the reproductive performance and egg biochemical composition were further studied for the evaluation of effects of fish oil replacement. The results showed that: (1) Feed 3# treatment started to spawn only after 10 days of post-mating, while there were 15 days of post-mating for the other treatments; the final ovigerous rate was 100% for the treatments of feed 1#, feed 3# and feed 5#, while the final ovigerous rate of feed 2# and feed 4# was around 80%; although feed 4# treatment had the highest egg production, fecundity and reproductive effort among five treatments, no significant differences were found among the five treatments(P>0.05). (2) As for the egg quality, feed 1# had the slightly higher egg diameter than the others,but no significant differences were found on egg diameter, individual egg wet weight and dry weight among the five treatment (P>0.05). (3) As for the egg biochemical composition, no significant differences were found for the moisture, crude protein, crude lipid and fatty acid composition in the eggs among the five treatments (P >0.05); the percentages of 18:2n6, and the ratio of n-6 polyunsaturated fatty acids/n-3 polyunsaturated fatty acids (∑n-6PUFA/∑n-3PUFA) increased with increasing the replacement levels of dietary vegetable oils, while the decreasing trend was found on the percentage of 22:6n3 in the eggs. In conclusion, dietary fish oil replacement have no significant effect on reproductive performance, egg quality, proximate composition, and fatty acid composition of eggs for feeding E. sinensis at middle stage of gonadal development. Therefore, the appropriate replacement level of dietary fish oil is around 75% in consideration of egg production and fecundity.
HTML 查看全文     下载PDF阅读器
关闭