文章摘要
张倩,丁洪昌,严兴洪,刘长军.皱紫菜色素突变体的分离与特性分析[J].上海海洋大学学报,2019,28(4):526-534
皱紫菜色素突变体的分离与特性分析
Isolation and characterization of pigmentation mutants of Pyropia crispata(Bangiales, Rhodophyta)
投稿时间:2018-05-02  修订日期:2018-08-08
DOI:10.12024/jsou.20180502305
中文关键词: 皱紫菜  紫外线  变异细胞  色素突变体  活体吸收光谱
英文关键词: Pyropia crispata  ultraviolet ray  color-mutated cell  pigmentation mutant  in vivo absorption spectrum
基金项目:国家高技术研究发展计划(2012AA10A411);国家自然科学基金(31072208);浙江省农业(水产)新品种选育重大科技专项(2016C02055-6)
作者单位E-mail
张倩 上海海洋大学 水产种质资源发掘与利用教育部重点实验室, 上海 201306
上海海洋大学 水产科学国家级实验教学示范中心, 上海 201306
上海海洋大学 上海水产养殖工程技术研究中心, 上海 201306 
 
丁洪昌 上海海洋大学 水产种质资源发掘与利用教育部重点实验室, 上海 201306
上海海洋大学 水产科学国家级实验教学示范中心, 上海 201306
上海海洋大学 上海水产养殖工程技术研究中心, 上海 201306 
 
严兴洪 上海海洋大学 水产种质资源发掘与利用教育部重点实验室, 上海 201306
上海海洋大学 水产科学国家级实验教学示范中心, 上海 201306
上海海洋大学 上海水产养殖工程技术研究中心, 上海 201306 
xhyan@shou.edu.cn 
刘长军 象山县水产技术推广站, 浙江 象山 315700  
摘要点击次数: 125
全文下载次数: 150
中文摘要:
      用不同剂量的紫外线(UV)对皱紫菜(Pyropia crispata)野生型品系(PC-WT)的壳孢子萌发体进行辐照,4周后,叶状体上均出现了黄绿、宝石红、黄橙等颜色的色素变异细胞块,经统计表明,剂量为80 μW/cm2的诱变效果最好。用酶解法将含有色素变异细胞块的叶状体细胞单个分离出来进行培养,从再生叶状体中分离到PC-JH(桔黄褐色)、PC-ZH(宝石红色)、PC-HL(黄绿色)、PC-QL(浅绿色)等纯色突变体,通过单性生殖分别获得纯合品系。各突变品系的F1叶状体的颜色和形态均一,且与其母体叶状体相同,表明各突变品系的颜色等突变性状均能稳定遗传。与野生型品系相比,各色素突变品系的F1叶状体的活体吸收光谱、色素蛋白的含量及相互间的比值均发生了明显变化,且相互之间也存在明显差异。综上所述,紫外线对皱紫菜叶状体具有良好的诱变效果,本实验所获得的色素突变品系,将成为皱紫菜遗传育种研究的重要材料。
英文摘要:
      The conchospore gametophytic blades of the wild-type strain of Pyropia crispata were irradiated with different doses of ultraviolet ray. After being cultured for about 4 weeks, many color-mutated cell clusters including yellow green, ruby red, yellow orange and so on,appeared on the irradiated blade. The statistical analysis showed most of the color-mutated cell types are yellow and the dose of 80 μW/cm2 attained the best mutagenic effect. Single mutated cell was isolated enzymatically from the color-mutated blades and was regenerated into blades, in which some single-color mutants were selected:PC-JH (orange brown), PC-ZH (ruby red), PC-HL (yellow green) and PC-QL (light green). Their homozygous strain were gotten by parthenogenesis, respectively. The color and morphology of the F1 gametophytic blades of each mutant strain showed uniform color and were same with its mother blade. The results indicated mutant characters such as color can be stably inherited in each mutant strain. Each mutant strain featured their own characteristics in in vivo absorption spectra of the blade, contents of chlorophyll a(Chl.a), phycocyanin(PC), phycoerythrin(PE) and their ratio, different from wild-type strain's. Compared with the wild-type strain (PC-WT), the content of PC and the value of PC/Chl.a of the two green-type strains(PC-HL, PC-QL) increased significantly, while the value of PE/PC decreased significantly and was close to one. The content of three pigment proteins in the orange-yellow mutant (PC-JH) was reduced, and the PC/Chl.a value was also lower, but the PE/Chl.a and PE/PC values were higher. The PE content of the date-red mutant (PC-ZH) increased significantly. Correspondingly, the values of PE/Chl.a and PE/PC also increased significantly. These results indicated that UV had a good mutagenic effect on Pyropia crispata blade, and the obtained four mutant strains were stable in color. They will become important materials for genetic breeding in Pyropia crispata.
HTML 查看全文     下载PDF阅读器
关闭