文章摘要
姚纪花,楼允东.三种群银鲫的RAPD分析初报[J].上海海洋大学学报,2000,(1):11-14
三种群银鲫的RAPD分析初报
Preliminary report on the RAPD analysis of three populations of Carassius auratus gibelio
  
DOI:
中文关键词: 银鲫 RAPD 种群鉴定 分子标记 遗传差异
英文关键词: Carassius auratus gibelio,population, RAPD
基金项目:农业部上海水产大学增养殖生态生理重点开放实验室资助!KFT1998-4号。
姚纪花  楼允东
上海水产大学渔业学院!200090
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中文摘要:
      利用20个随机引物对产于方正、彭泽和淇河地区的三个银鲫种群进行了RAPD检测结果显示14个引物的扩增效果良好,单一引物扩增的DNA片段数在2 ̄13条之间,片段长度在300 ̄4000bp之间。根据RAPD结果,进行了银鲫种群内、种群间的遗传差异分析。14个引物中,引物OPV-07、OPV-14和OPV-20等对三种群银鲫扩增出的指纹图谱差异显著,可作为银鲫种群鉴定的分子标记。
英文摘要:
      populations of Carassius auratus gibelio lived in Fangzheng County (Heilongjiang Province), Pengze County (Jiangxi Province) and Qihe River (Henan Province) were analyzed by RAPD method using twenty arbitrary primers. The experiment shows that fourteen primers were effective, each primer gave 2 - 13 bands for the each individual. The length of the fragment was 300 - 4000bp. According to the patterns, genetic variations of inter-and intrapopulations of C. auratus gibelio were analysed. Among three populations, the differences of fingerprints obtained by primers OPV - 07, OPV - 14 and OPV - 20 were so obviously that might be used to be molecular markers.
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