文章摘要
郭亚贞,王慥,曲鹏鹏.香菇多糖的纯化和结构分析[J].上海海洋大学学报,2000,(1):35-39
香菇多糖的纯化和结构分析
Purification and identification of polysaccharide from Lentinus edodes
  
DOI:
中文关键词: 香菇 多糖 结构分析 提取 酶法 产率
英文关键词: Lentinus edodes, polysaccharide, lentinan, purification
基金项目:
郭亚贞  王慥  曲鹏鹏
[1]上海水产大学食品学院 [2]上海市食品研究所食品工艺与包装研究室
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中文摘要:
      香菇中提取到的水溶性粗多糖,用Sevag法结合酶法去蛋白,透析,甲醇分级得到2个多糖级分,分别命名为Len1和Len2。去蛋白后多糖经Sephadex G-100凝胶过滤层析纯化得精品多糖Len3,经纸层析及凝胶过滤层析表现单一区带或单一峰。经酸水解后纸层析和气相色谱分析表明Len2含甘露糖、葡萄糖,摩尔比为甘露糖:葡萄糖=10.50:1.00,Len3含木糖、甘露糖、葡萄糖,摩尔比为木糖:甘露糖:葡萄糖=0.67:2.45:1.00。200 ̄400nm紫外扫描,无核酸和蛋白吸收峰。4000 ̄650cm^-1红外光谱扫描,呈多糖类特征吸收峰。
英文摘要:
      Water soluble polysaccharide (lentinan) was crudely extracted from Lentinus edodes, then purified by the methanol fractionation with polysaccharide solution: methanol(v/v) 1: 0. 5 and 1: 1 respectively named ben 1 and ho 2. subsequently, the deproteinized polysaccharide was refined by gel filtration with Sephadex G-100 named Len 3. The finished products were identified by paper chromatography and gel filtration with Sephadex G-100. The compositions of ten 2 and Len 3 were separated by paper-and gas-chromatography. The results show that Len 2 is composed of mannose and glucose with a molar ratio of 10. 50: 1. 00, but Len 3 is composed of xylose, mannose and glucose with a molar ratio of 0.67:2 .45: 1 .00.
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