文章摘要
张殿昌,江世贵,苏天凤,蔡云川,许新萍.鲮胰岛素样生长因子Ⅰ(IGF-Ⅰ)cDNA的分子克隆和序列分析[J].上海海洋大学学报,2002,(2):97-101
鲮胰岛素样生长因子Ⅰ(IGF-Ⅰ)cDNA的分子克隆和序列分析
Molecular cloning and sequence analysis of insulin-like growth factor I(IGF-I)cDNA from Cirrhinus molitorella
  
DOI:
中文关键词: 鲮 胰岛素样生长因子I(IGF-I) 分子克隆
英文关键词: Cirrhinus molitorella,insulin like growth factor I (IGF I),molecular cloning
基金项目:
张殿昌  江世贵  苏天凤  蔡云川  许新萍
[1]中国水产科学院研究南海水产研究所,广东广州510300 [2]中山大学生命科学学院,广东广州510275
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中文摘要:
      采用逆转录-聚合酶链式反应(RT-PCR)方法,从鲮肝脏总RNA中扩增出胰岛素样生长因子-I(IGF-I)基因,克隆至质粒PUGm-T。测定该基因序列,推导其编码的蛋白质序列。克隆的鲮IGF-IcDNA编码序列包括信号肽、B、C、A、D和E6个区域,共161个氨基酸残基。与鲤IGF-I比较,信号肽由44个氨基酸残基组成比鲤少17个,成熟肽核苷酸序列和氨基酸序列的同源性分别为95.2%和100%,E区域分析结果表明,克隆的鲮IGF-I序列属于IGF-I Ea-2亚型。
英文摘要:
      Total RNA was isolated from Cirrhinus molitorella liver tissue. The cDNA encoding insulin like growth factor I (IGF I) was amplified by reverse transcription polymerase chain reaction (RT PCR) strategy using isolated total RNA as template.The amplified cDNA fragment was inserted to vector pUCm T. The cloned cDNA was sequenced and the amino acid sequence of Cirrhinus molitorella IGF I was predicted .The nucleotide sequence and deduced amino acid sequence shared 95.2% and 100% homology with that of Cyprinus carpio mature IGF I peptide. Analysis of E domain indicated that cloned Cirrhinus molitorella belonged to IGF I Ea 2 subtype.
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