文章摘要
刘艳红,肖调义,苏建明,邱高峰.用显微注射法将绿色荧光蛋白基因导入金鱼受精卵中表达[J].上海海洋大学学报,2002,(2):102-105
用显微注射法将绿色荧光蛋白基因导入金鱼受精卵中表达
Expression of GFP gene transferred into the fertilized eggs of Carassius auratus by microinjection
  修订日期:2002-03-20
DOI:
中文关键词: 金鱼 绿色荧光蛋白基因 转基因鱼 显微注射
英文关键词: Carassius auratus,green fluorescent protein (GFP) gene,transgenic fish,microinjection
基金项目:
刘艳红  肖调义  苏建明  邱高峰
[1]上海水产大学渔业学院,上海200090 [2]湖南农业大学动物科技学院,长沙410128
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中文摘要:
      采用显微注射法将绿色荧光蛋白(GFP)基因重组表达质粒导入金鱼受精卵中,以期获得能发绿色荧光的金鱼。结果显示,在注射的3批金鱼受精卵中(第一批4310粒,第二批3952粒,第三批4056粒),分别孵出鱼苗543、282和266尾,出苗率为17.02%、12.53%和13.52%,其中表达绿色荧光的金鱼分别为23、24和18尾,表达率为4.24%、8.51%和6.77%。荧光表达检测发现,从肌肉效应期就开始检测得到绿色荧光。
英文摘要:
      In order to obtain Carassius auratus with green fluorescence, the plasmid GFP gene was transferred into the fertilized eggs by microinjection. The results showed that the three batches of fertilized eggs (4310,3952, 4056) hatched 543?282 and 266 fry, respectively, and the survival rates were 17.02%?12.53% and 13.52%, respectively; there were 23?24 and 18 fry with green fluorescence, respectively, and the expression rates were 4.24% ?8.51% and 6.77%, respectively. The detection of green fluorescence indicated that GFP gene begins to express in the muscular effect stage of gold fish embryos.
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