文章摘要
李家乐,钱荣华,鲍宝龙,汪桂玲,戚鸟定.中国五大湖三角帆蚌群体遗传多样性的RAPD分析[J].上海海洋大学学报,2005,(1):1-5
中国五大湖三角帆蚌群体遗传多样性的RAPD分析
RAPD analysis on genetic diversity among the stocks of Hyriopsis cumingii from the five large lakes of China
  修订日期:2005-01-06
DOI:
中文关键词: 五大湖,三角帆蚌,群体,RAPD
英文关键词: the five large lakes,Hyriopsis cumingii,stock,random amplified polymorphic DNA
基金项目:上海市科委基础重点项目(03JC14063),上海市教育基金会曙光计划项目(01SG42)
李家乐  钱荣华  鲍宝龙  汪桂玲  戚鸟定
上海水产大学农业部水产种质资源与养殖生态重点开放实验室 上海 200090 (李家乐,钱荣华,鲍宝龙,汪桂玲)
,浙江七大洲珠宝有限公司 浙江诸暨 311804(戚鸟定)
摘要点击次数: 876
全文下载次数: 738
中文摘要:
      用OPJ和OPM两组40条10碱基随机引物,对中国五大湖三角帆蚌地理群体及诸暨养殖蚌进行了随机扩增多态性DNA(RAPD)分析,其中12个引物的扩增结果具有丰富的群体多态性,多态率为55.6%~80%。群体内遗传相似度大小依次为:鄱阳湖(0.8889),太湖(0.8694),洞庭湖(0.8111),诸暨(0.7746),洪泽湖(0.7348),巢湖(0.7185)。依据群体间遗传距离指数及分子系统树,表明洞庭湖群体和洪泽湖群体亲缘关系最近,鄱阳湖群体则与巢湖群体的亲缘关系最近,并且诸暨人工养殖群体与鄱阳湖和巢湖的群体比较接近。
英文摘要:
      With the random amplified polymorphic DNA (RAPD) analysis method, forty ten-base arbitrary primers of OPJ and OPM groups were used to study the genetic variability among geographic stocks of Hyriopsis cumingii from the five large lakes of China: Po-yang Lake (PY), Dong-ting Lake (DT), Tai Lake(TH), Chao Lake (CH) and Hong-ze Lake (HZ), and one cultured stock from Zhuji City (ZJ) of Zhejiang Province. The amplified results of twelve primers showed abundant polymorphy, and the percentage of polymorphy with each primer amplified varied 55.6% to 80%. The intra-stock genetic similarity indexes ranked as PY(0.888 9)>TH (0.869 4)> DT ((0.811 1))> ZJ (0.774 6)> HZ(0.734 8)> CH(0.718 5). Based on inter-stock genetic distance indexes and molecular phylogenetic tree, we can conclude that the phylogeny relation between DT and HZ stocks is closest, and the cultured stock from ZJ is relatively close to the cluster of PY and CH stocks.
HTML 查看全文     下载PDF阅读器
关闭