文章摘要
张传涛,李思发,颉晓勇.吉富品系尼罗罗非鱼选育系F6~F9遗传变异的RAPD分析[J].上海海洋大学学报,2007,(1):7-10
吉富品系尼罗罗非鱼选育系F6~F9遗传变异的RAPD分析
RAPD analysis of genetic variation of GIFT strain Oreochromis niloticus from 6th to 9th generation
投稿时间:2006-02-26  
DOI:
中文关键词: 尼罗罗非鱼  选育  世代  遗传变异
英文关键词: RAPD
基金项目:国家科技攻关计划;国家科技攻关计划
张传涛  李思发  颉晓勇
上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090
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中文摘要:
      利用随机扩增多态DNA(RAPD)技术对连续选育的吉富品系尼罗罗非鱼F6,F7、F8、F9R4个世代群体的遗传变异进行了分析。结果表明:(1)17条引物在4个世代的88尾个体间共检测到172个位点。(2)F6, F7,F8、F94世代的多态座位比例分别为:23.4%、21.1%、19.4%、18.3%,呈递减状态,U检验差别不显著(P〉0.05);4世代的Nei遗传相似系数分别为:O.8961、0.9084,0.9186、0.9254,呈递增状态,t检验差别不显著(P〉0.05);Shannon多样性指数分别为:0.1485、0.1314,0.1178、0.1083,呈递减状态,t检验差别不显著(P〉0.05)。(3)4世代间的遗传变异占群体总变异的比例是23.9%(Shannon多样性指数分析)和12.5%(AMOVA分析)。以上结果表明,4代选育虽未导致选育群体遗传多样性产生显著的差异,但却清楚地显示了逐代选育使选育系群体趋于纯化.选育系F8、F9世代已表现出较好的遗传稳定性。
英文摘要:
      Random Amplified Polymorphic DNA (RAPD) was used to detect the variation of four successively selected generations( F_6,F_7,F_8,F_9 ) of GIFT strain of Nile tilapia(Oreochromis niloticus).The results showed that: (1)172 loci (300-2800 bp ) were examined by 17 random primers from 88 individuals of four generations. (2)The proportions of polymorphic loci of F_6,F_7,F_8,F_9 were 23.4%,21.1%,19.4%and 18.3% respectively,decreased generation by generation,but no significant differences among generations (U test, P>0.05);the Nei genetic identities of F_6,F_7,F_8,F_9 were 0.896 1,0.908 4,0.918 6,0.925 4 respectively ,increased generation by generation,but no significant differences among generations(t- test, P>0.05);Shannon genetic diversity indices of F_6,F_7,F_8,F_9 were 0.148 5,0.131 4,0.117 8,0.108 3, decreased generation by generation, but no significant differences among generations(t -test, P>0.05).(3)The contribution of the variation among four generations to the total variations of four generation was 23.1% in Shannon genetic diversity index and 12.5% in AMOVA analysis. The above results showed that, although a selection of four generations did not cause a significant difference of genetic diversities, it caused a clear trend in genetical purification, and the F_8 and F_9 generations have reached a rather high genetic stability as a genetic improved variety.
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