文章摘要
王燕飞,徐义平,鲍宝龙.斑马鱼HCS基因全长cDNA序列及早期仔鱼阶段的空间表达[J].上海海洋大学学报,2013,22(3):320-327
斑马鱼HCS基因全长cDNA序列及早期仔鱼阶段的空间表达
Cloning and expression of HCS gene in zebrafish larvae
  
DOI:
中文关键词: 羧化全酶合成酶  斑马鱼  生物素酰化  RNA原位杂交
英文关键词: holocarbonxylase synthetase  zebrafish  biotinylation  RNA in situ hybridization
基金项目:教育部重点项目(211056);上海市教育委员会创新项目(11ZZ146)
作者单位
王燕飞 上海海洋大学 农业部淡水水产种质资源重点开放实验室 
徐义平 上海海洋大学 农业部淡水水产种质资源重点开放实验室 
鲍宝龙 上海海洋大学 农业部淡水水产种质资源重点开放实验室 
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中文摘要:
      蛋白生物素酰化发挥重要的生理作用,羧化酶和组蛋白生物素酰化主要依赖于羧化全酶合成酶(holocarboxylase synthetase,HCS)。首次克隆了HCS基因全长序列2 942 bp,其编码829个氨基酸,含有BirA功能结构域。利用RNA整体原位杂交技术,检测HCS基因在斑马鱼早期发育过程中的空间表达,发现HCS基因在脑、脊髓、鳃、心脏、肠道和肌肉等组织中表达。在肌肉组织中,HCS基因在初孵仔鱼的肌节表达,而在孵化后第6天仔鱼中,HCS基因则集中在每一肌节的中部表达。这些结果,为进一步了解HCS的功能奠定了基础。
英文摘要:
      Some proteins or enzymes play physiologic roles depending on its biotinylation via catalyzation of holocarboxylase synthetase (HCS), such as histones and carboxylases. So far, our understanding of HCS function is mainly from the researches on human cell lines. In order to understand the role of protein biotinylation in fish development, we cloned full length HCScDNA sequence with 2 942 bp. Intergrity open reading frame encodes 829 amino acids, containing conserved BirA ligase domain close to C termino. Further investigation using whole mount RNA in situ hybridization shows that HCSgene is expressed in wide tissues such as brain, spine cord, gill, heart, intestine and sarcomere, indicating that it might play universal roles during organs development. It is interesting that HCS gene is expressed in whole sarcomere at new hatching larvae, whereas at 6 DAH (day after hatching) larvae, expression signal of HCSgene concentrated on the middle of each sarcomere. Above results may be helpful for future studies on HCS function during fish development.
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