文章摘要
王 宁,陆宏达,余伟楠.中华绒螯蟹体表的钟形钟虫重描述及其系统发育地位分析[J].上海海洋大学学报,2013,22(4):609-615
中华绒螯蟹体表的钟形钟虫重描述及其系统发育地位分析
Redescription and phylogenic analysis of Vorticella campanula attached on Eriocheir sinensis
  
DOI:
中文关键词: 中华绒螯蟹  钟形钟虫  重描述  系统发育  SSU rRNA
英文关键词: Eriocheir sinensis  Vorticella campanula  redescription  phylogenic  SSU rRNA
基金项目:上海市农委中华绒螯蟹产业技术体系建设项目(D8003100208);上海海洋大学研究生科研基金项目(A2500110033);上海高校知识服务平台上海海洋大学水产动物遗传育种中心项目(ZF1206)
作者单位
王 宁 上海海洋大学 水产与生命学院 
陆宏达 上海海洋大学 水产与生命学院 
余伟楠 上海海洋大学 水产与生命学院 
摘要点击次数: 1198
全文下载次数: 1102
中文摘要:
      通过活体观察、蛋白银染色法和硝酸银染色法对附生于中华绒螯蟹(Eriocheir sinensis)上的一种钟形钟虫(Vorticella campanula)的形态学进行了重描述,并且补充了该虫未曾描述过的一些形态学特征,这些补充的特征为:伸缩泡一个,直径为7.5~8.7 μm;胞质内分布着自由运动的黄色、绿色、褐色及黑色小颗粒;直径为 9~15 μm的圆形食物泡;茎宽为5.1~7.6 μm;茎内肌丝宽为2.7~3.2 μm,肌丝上密集分布着直径为0.5~0.7 μm的黑色小颗粒;虫体表膜外侧明显横纹,内侧颗粒状;小膜3外侧2列动基较内侧动基长,小膜2的3列动基等长且终止于小膜1和小膜3的交汇处,小膜1内侧2列动基较外侧动基长;嗜银颗粒明显、密集且分布不均。对钟形钟虫SSU rRNA序列的分子生物学分析对比,与Vorticella campanula(JN120250)的相似率为99.61%。系统发育分析表明,钟形虫属与螅状独缩虫亲缘关系较近,钟形钟虫比同属中其他钟形虫更原始,较早地从钟形虫属中分化了出来。
英文摘要:
      The morphological characteristics of Vorticella campanula attached on Eriocheir sinensis were redescribed by the observations of living bodies and protargol and silver nitrate impregnated bodies. Some new morphological supplemented characteristics were: One contractile vacuole was 7.5-8.7 μm in diameter. Freedom movements of some yellow, green, brown and black granules were within the protoplasm. Food vacuoles were 9-15 μm in diameter. Stalks were 5.1-7.6 μm in width. Myonemes were 2.7-3.2 μm in width, on which some black granules in the diameter of 0.5-0.7 μm were densely distributed. The clear striaes were on the outside of pellicle and the granulars were in the inside of pellicle. The outer two kineties were longer than the inner kinety of P3. The three kineties of P2 were equal in length and end of junction of P1 and P2. The outer two kineties were longer than the inner kinety of P1. The argyrophilic grains were obvious and densely and unevenly distributed. Phylogenetic analysis indicates that Vorticella campanula was more original than other Vorticella campanula and was divided from Vorticella at the earliest stage.
HTML 查看全文     下载PDF阅读器
关闭