文章摘要
孙丽慧,沈斌乾,陈建明,潘 茜,郭建林.日本沼虾对饲料赖氨酸的需要量研究[J].上海海洋大学学报,2013,22(1):
日本沼虾对饲料赖氨酸的需要量研究
Studies on dietary lysine requirement of Macrobrachium nipponense
投稿时间:2012-06-08  修订日期:2012-07-31
DOI:
中文关键词: 日本沼虾,饲料,赖氨酸,需要量,虾体增重
英文关键词: Macrobrachium nipponense  dietary lysine  requirement  growth performance
基金项目:浙江省重大国际合作项目(2009C14019);湖州市水产养殖创新团队计划(2010KC02);浙江省淡水养殖创新团队计划(2012R10026-09)
作者单位E-mail
孙丽慧 浙江省淡水水产研究所 浙江湖州 313001 miduo1984422@126.com 
沈斌乾 浙江省淡水水产研究所 浙江湖州 313001  
陈建明 浙江省淡水水产研究所 浙江湖州 313001 aqua_labjm@163.com 
潘 茜 浙江省淡水水产研究所 浙江湖州 313001  
郭建林 浙江省淡水水产研究所 浙江湖州 313001  
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中文摘要:
      在10%酪蛋白和5%明胶提供全蛋白氨基酸基础上,用晶体氨基酸赖氨酸调节7个饲料赖氨酸水平(0.85%~2.95%),并以晶体天冬氨酸和谷氨酸(1:1)补偿调节百分比;其它饲料氨基酸分别用晶体氨基酸调节到40%的日本沼虾肌肉蛋白中各氨基酸含量的水平,配制7种精制试验饲料,喂养初始体重为0.13g的幼日本沼虾7周。结果表明,用不同试验饲料喂养日本沼虾7周后,虾的存活率组间无显著差异(P>0.05)。饲料赖氨酸水平对虾体增重有显著影响(P<0.05)。虾体增重随饲料赖氨酸上升到1.90%不断增大,但超过此值后不再有显著变化。摄食饲料赖氨酸水平为1.55%~2.60%的试验组的饲料效率显著高于摄食饲料赖氨酸水平为0.85%的试验组。以虾体增重为指标,经折线模型回归分析,日本沼虾对赖氨酸的需求量为:占干饲料的1.91%,占饲料蛋白的5.41%。并以此为基准,以肌肉必需氨基酸组成比为参考,估算了其它各必需氨基酸的需要量分别为:精氨酸1.83%、组氨酸0.52%、异亮氨酸1.13%、亮氨酸1.80%、蛋氨酸0.66%、苯丙氨酸0.99%、苏氨酸0.81%、色氨酸0.27%、缬氨酸1.11%。
英文摘要:
      7 purified diets with various lysine levels ranging from 0.85% to 2.95% were formulated by using crystal amino acids and intact protein amino acids from 10% casein and 5% gelatin. Levels of crystal lysine were graded and the percentage differences were compensated by levels of mixture of aspartic acid and glutamate (1:1) accordingly. Levels of other amino acids were maintained to analyzed ones in 40% protein of tail muscle. The diets were fed to 7 triple groups of Macrobrachium nipponense juveniles initially weighing 0.13g for 7 weeks. The survival rates had no significantly differences (P>0.05) between treatments, while the weight gains of shrimps fed diets with various levels of lysine were significantly affected (P<0.05). Weight gain increased with increasing dietary lysine levels from 0.85% to 1.90%, and further increase dietary lysine level seemed to reach a platform. Feed efficiencies of shrimp fed diets with lysine levels ranging from 1.55% to 2.60% were significantly higher than that of shrimp fed a diet with 0.85% lysine (P<0.05). Broken line model regressions analysis of weight gain data showed that the dietary lysine requirement of the shrimp was 1.91% of dry diet or 5.41% of dietary protein. And based on this result, the recommended requirements for other 9 essential amino acids were calculated by using a reported essential amino acids profile through analyzing the tail muscle.
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