文章摘要
王 元,房文红,周俊芳,李新苍,刘 强.“脊尾白虾肌肉微孢子虫病”的病原和病理分析[J].上海海洋大学学报,2013,22(5):
“脊尾白虾肌肉微孢子虫病”的病原和病理分析
The pathogenic and pathological analysis of “microsporidiasis in the muscle of Exopalaemon carinicauda”
投稿时间:2013-03-13  修订日期:2013-05-30
DOI:
中文关键词: 脊尾白虾  微孢子虫  包囊  超微结构  超微病理
英文关键词: Exopalaemon carinicauda  microsporidian  sporophorous vesicle  ultrastructure  ultrastructural pathology
基金项目:公益性行业(农业)科研专项基金(201303047).
作者单位E-mail
王 元 中国水产科学研究院东海水产研究所 上海海洋大学 wy9501@yeah.net 
房文红 中国水产科学研究院东海水产研究所  
周俊芳 中国水产科学研究院东海水产研究所 zhoujf@eastfishery.com.cn 
李新苍 中国水产科学研究院东海水产研究所  
刘 强 中国水产科学研究院东海水产研究所 上海海洋大学  
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中文摘要:
      江苏启东海水养殖脊尾白虾出现肌肉白化症状,虾腹部、头胸甲、步足、尾扇等部位病变明显,通过活体压片镜检发现肌肉中存在大量球形包囊,利用超显微技术对病原的形态和结构进行分析,确定其为微孢子虫,并对脊尾白虾的病变组织进行了病理分析。结果表明:该微孢子虫具包囊结构,包囊大小为5.4±0.55(4.4~6.6)μm,一个包囊内含8个孢子,孢子呈椭圆形,成熟孢子大小为2.3±0.25(1.7~2.8)μm×1.5±0.19(1.2~1.9)μm,孢内极丝一般环绕9~10圈。病理分析显示,脊尾白虾的鳃、胃、肠和肌肉内均发现微孢子虫寄生和不同程度的病变。其中,肌肉组织病变最严重:原肌纤维存在的空间被大量的球形包囊占据,肌纤维断裂,空泡数量增多,线粒体消失;鳃细胞膨大呈空泡状,鳃膜坏死,核质、细胞质和细胞器消失;胃细胞胞浆基质电子密度增大,孢子寄生在结缔组织中脂滴附近;肠外膜上皮细胞核质皱缩,部分肌层消失,孢子寄生在肠粘膜层和肌层之间。
英文摘要:
      The symptom of muscle whitish occurred in marine cultured ridgetail white prawn, Exopalaemon carinicauda, in Qidong, Jiangsu province. The muscle of abdomen, cephalothorax, pereiopoda, tail fan showed clearly pathological changes. The microscopical examination with wet mount of vivo revealed many spherical cysts existed in muscle tissues. Morphological and structural analysis of pathogen confirmed it was the microsporidian by using ultramicroscopic technique. The pathological analyses of infected tissues were also performed. The results indicated that this microsporidian was featured by sporophorous vesicle structure. Sporophorous vesicles containing eight spores measured 5.4 ± 0.55 (4.4~6.6) μm. The mature spores were oval-shaped, approximately 2.3±0.25 (1.7~2.8) μm ×1.5±0.19 (1.2~1.9) μm with 9-10 polar ?lament coils. Pathological analysis showed that the parasite invaded the gill, stomach, gut and muscle tissues of host and caused different pathological changes, especially worst in the skeletal muscle. Muscle fibres of the abdomen were largely replaced by spherical sporophorous vesicles. Myofibres were ruptured apparently. The number of vacuoles increased and the mitochondria disappeared. The hypertrophy and vacuolization, part of gill membranes necrosis, disappearing of nucleoplasm, cytoplasm and organelles were observed in gill cells. The electron densities of cytoplasmic matrix in the stomach cells increased and the spores lived around the lipid droplets in connective tissue. The nucleoplasm of the gut epithelial cell condensed and some muscularis disappeared. Parasites located between the submucosa and muscle layer in gut.
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