文章摘要
蒋飞,戴习林,朱其建,过正乾,苏建,鞠波,丁福江.罗氏沼虾5个专门化品系选择系生长比较及其遗传结构分析[J].上海海洋大学学报,2014,23(3):329-337
罗氏沼虾5个专门化品系选择系生长比较及其遗传结构分析
Comparison of growth performance and analysis of genetic structure of 5 specialized strains of Macrobrachium rosenbergii
投稿时间:2014-01-22  修订日期:2014-03-17
DOI:
中文关键词: 罗氏沼虾  专门化品系选择系  生长  遗传多样性
英文关键词: Macrobrachium rosenbergii  specialized strains  growth  genetic diversity
基金项目:上海市科学技术委员会重点攻关项目(11391901400);上海市科技兴农重点攻关项目[沪农科攻字(2010)第1-6号];上海市教育委员会重点学科建设项目(J50701)
作者单位E-mail
蒋飞 上海海洋大学 水产与生命学院, 上海 201306  
戴习林 上海海洋大学 水产与生命学院, 上海 201306 xldai@shou.edu.cn 
朱其建 上海海洋大学 水产与生命学院, 上海 201306  
过正乾 上海海洋大学 水产与生命学院, 上海 201306  
苏建 上海海洋大学 水产与生命学院, 上海 201306  
鞠波 上海海洋大学 水产与生命学院, 上海 201306  
丁福江 上海申漕特种水产开发公司, 上海 201516  
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中文摘要:
      采用单因素完全随机设计和荧光标记混养两种实验方法,对罗氏沼虾5个专门化品系选择系A、B、C、D、E的子二代进行生长性能测定及比较分析。结果显示无论是单因素完全随机实验还是荧光标记混养实验,各选择系子二代的绝对增重率和特定增重率的大小顺序依次为:D>B>E>C>A;经方差分析和Duncan氏法多重比较,选择系B和D的子二代表现出明显的生长优势。子二代中符合各选择系的体型比例较子一代有所增加;子二代的体重变异程度比子一代的体重变异程度小,其中B降幅最为明显,高达49.22%,说明子二代规格相对于子一代更加整齐。应用微卫星分子标记对罗氏沼虾5个选择系子二代共630尾虾的遗传多样性和遗传分化进行了研究,分析表明:各位点的平均等位基因数为6.25;平均有效等位基因数为3.666 2;平均杂合度观测值为0.435 0,平均杂合度期望值为0.707 6。5个选择系的多态信息含量(PIC)从小到大依次为C(0.434 0)GST)为0.074 5,大部分遗传变异来源于各选择系内,表明各选择系尚具有进一步选育的潜力。
英文摘要:
      Growth performance of F2 of 5 specialized strains of Macrobrachium rosenbergii(A,B,C,D and E) was checked by one-way completely randomized design and polyculture in concrete ponds with the fluorescent tag. The results indicated that the ranks of AGR and SGR were D>B>E>C>A in either one-way completely randomized experiment or polyculture in concrete ponds with the fluorescent tag. AGR and SGR by ANOVA and Duncan for strains of multi-average revealed that F2 of B and D were strains with the best growth traits.Every proportion of body form of F2 was greater than F1; Variation coefficient of body weight of F2 was lower than F1, the most obvious decline in the comparison of F2 and F1 was B, decreasing 49.22%.All of them suggested preliminary benefit of strain selecting.Microsatellite markers were used to analyze the genetic diversity and genetic differentiation of F2 of 5 specialized strains, including 630 individuals. The results showed the allele number was 6.25; average effective allele number was 3.666 2; average observed heterozygosity value was 0.435 0; the average expected heterozygosity value was 0.707 6. All of these five specialized strains in the ascending order the PIC was C(0.434 0)< A(0.480 4)
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