文章摘要
潘桂平,刘本伟,周文玉.低温胁迫对云纹石斑鱼幼鱼抗氧化和免疫指标的影响[J].上海海洋大学学报,2016,25(1):78-85
低温胁迫对云纹石斑鱼幼鱼抗氧化和免疫指标的影响
The effects of cold stress on the antioxidant defense and immune parameters of juvenile Epinephelus moara
投稿时间:2015-04-11  修订日期:2015-07-23
DOI:10.12024/jsou.20150401396
中文关键词: 云纹石斑鱼  幼鱼  低温胁迫  抗氧化保护  免疫性能
英文关键词: Epinephelus moara  juvenile  low temperature stress  antioxidant defense  immunity
基金项目:上海市科技兴农重点攻关项目[沪农科攻字(2013)第6-3号、沪农科攻字(2009)第5-6号]
作者单位E-mail
潘桂平 上海市水产研究所, 上海 200433  
刘本伟 上海市水产研究所, 上海 200433  
周文玉 上海市水产研究所, 上海 200433 wyzh265@163.com 
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中文摘要:
      低温是鱼类常见的环境胁迫因子之一,有关云纹石斑鱼对低温胁迫的生理响应尚未见报道。本实验设置了9、13、17 ℃ 3 个温度梯度,研究短期低温胁迫(7 d)对云纹石斑鱼幼鱼肝脏和血清中抗氧化和免疫指标的影响。结果显示,肝脏中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化酶(GSH-Px)随胁迫温度的降低和胁迫时间呈上升趋势;不同温度组,肝脏中总抗氧化能力(T-AOC)和丙二醛(MDA)含量变化趋势不同,胁迫过程中9 和13 ℃组均为先上升后下降趋势,17 ℃组无显著变化。9 ℃组血清中SOD和T-AOC在7 d胁迫前后显著上升,而其MDA含量在胁迫前后显著下降,其余抗氧化指标变化不显著。就血清免疫指标而言,各温度组血清中溶菌酶(LZM)活力和免疫球蛋白M(Ig M)含量在低温胁迫条件下均呈先下降后上升趋势,但Ig M含量变化并不显著。综上,低温胁迫对云纹石斑鱼幼鱼抗氧化和免疫性能影响显著,低温胁迫导致该鱼体内的MDA含量升高,因而肝脏合成更多的抗氧化物质来进行抗氧化防御。
英文摘要:
      Low water temperature is one of the common enviromental stressors for fishes, but no available information could be found on the physiological response to low water termperature for Epinephelus moara. The current study was conducted to investigate the effects of low water temperature stress ( 0-7 days) on the activities of antioxidant enzymes and immune parameters in serum and liver of juvenile E moara, and three water temperatures were set at 9, 13 and 17 ℃,respectively. The result demonstrated that the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) increased with the decreasing water temperature and the increasing stress time. The total antioxidant capacities (T-AOC) and malondialdehyde (MDA) contents showed different trends in the livers of the different temperature treatments, i.e., the trends of “increasing-decreasing” were found in the livers from 9 and 13 ℃ treatments while no significant difference was found in the liver of 17 ℃ treatment. The significant differences were detected in the serum T-AOC and SOD from the beginning to the end of the experiment, and the significant decrease was found on the MDA in the serum. However, the other parameters were not related significantly to the low temperature. As for immune indices in the serum, the trends of “high-low-high” were found in lysozyme (LZM) activities and contents of immunoglobulin M (IgM), but no significant difference was found on the Ig M. In conclusion, the low water temperature stress had the significant effects on the antioxidant and immune abilities for juvenile E. moara, and the low temperature led to the increasing MDA in E. moara, which may induce the synthesis of some antioxidant in the liver.
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