文章摘要
李泽滨,李云,蔡生力,鞠晨曦.温度与光照周期对公子小丑鱼幼鱼生长及相关生长基因表达的影响[J].上海海洋大学学报,2017,26(3):321-329
温度与光照周期对公子小丑鱼幼鱼生长及相关生长基因表达的影响
Effects of different temperatures and photoperiods on growth and expressions of growth-related genes of young Amphiprion ocellaris
投稿时间:2016-03-21  修订日期:2017-02-17
DOI:10.12024/jsou.20160301699
中文关键词: 公子小丑鱼  温度  光照周期  生长  生长激素受体  类胰岛素生长因子  类胰岛素生长因子结合蛋白  基因表达
英文关键词: Amphiprion ocellaris  temperature  photoperiod  growth  GHR  IGF  IGFBP  gene expression
基金项目:上海海洋大学科技发展专项基金(A2020915200056)
作者单位E-mail
李泽滨 上海海洋大学 水产与生命学院, 上海 201306  
李云 上海海洋大学 水产与生命学院, 上海 201306 liyun@shou.edu.cn 
蔡生力 上海海洋大学 水产与生命学院, 上海 201306  
鞠晨曦 上海海洋大学 水产与生命学院, 上海 201306  
摘要点击次数: 1175
全文下载次数: 1520
中文摘要:
      探究了不同温度、光照周期对公子小丑鱼(Amphiprion ocellaris)幼鱼成活率、饵料转化率及生长率的影响,并利用实时荧光定量PCR技术检测了50 d后幼鱼肝脏生长激素受体基因Ⅰ型(Growth hormone receptor 1)、生长激素受体基因Ⅱ型(Growth hormone receptor 2)、类胰岛素生长因子基因(Insulin-like growth factor)和类胰岛素生长因子结合蛋白基因(Insulin-like growth factor-binding protein)相对表达量的变化情况。结果显示,不同温度、光照周期对公子小丑鱼幼鱼的成活率无显著影响(P>0.05),但对其生长率、饵料转化率影响显著。幼鱼的生长率、饵料转化率在温度27℃时最大,22℃次之,17℃、32℃较低;光照周期方面,幼鱼的生长率、饵料转化率在 24L:0D组最大,且显著高于其他光照周期组。荧光定量PCR的结果显示,幼鱼肝脏各生长相关基因的相对表达量在温度27℃时最高,且显著高于其他温度组(P<0.05);光照周期方面,各基因在 24L:0D时相对表达量最高,且显著高于其他光照周期组(P<0.05)。研究结果表明,公子小丑鱼幼鱼生长的最适温度为27℃,最适光照周期为 24L:0D。
英文摘要:
      The effects of different temperatures and photoperiods were studied on growth, feed conversion rate and relative expressions of growth hormone receptor Ⅰ (GHR1),growth hormone receptor Ⅱ (GHR2),insulin-like growth factor (IGF)and insulin-like growth factor binding protein (IGFBP) of young Amphiprion ocellaris.The results showed that there was no significant difference among the survival rate of young Amphiprion ocellaris under different temperature and photoperiod conditions(P>0.05). The growth rate and feed conversion rate of fish in 27℃ group were higher than those in other groups. In the photoperiod test, the growth rate and feed conversion rate of fish in 24L:0D group were significantly higher than those in other groups(P<0.05). At the end of the experiments, the expressions of several growth-related genes were investigated. The fish in 27℃ group showed the highest expressions of all the genes investigated(P<0.05). In the photoperiod test, the fish in 24L:0Dgroup showed the highest expressions of all the genes investigated(P<0.05).These results indicated that the most suitable temperature and photoperiod for growth were 27℃ and 24L:0D.
HTML 查看全文     下载PDF阅读器
关闭