文章摘要
张志允,李思发,蔡完其,谢中富,殷黎明.中华鳖黄河群体选育世代F1、F2及F3遗传变异微卫星分析[J].上海海洋大学学报,2011,20(2):161-166
中华鳖黄河群体选育世代F1、F2及F3遗传变异微卫星分析
Microsatellite analysis of genetic variation in F1,F2 and F3 selected generations of Yellow River populations of Chinese soft shelled turtle, 〖WTHX〗Trionyx sinensis
  
DOI:
中文关键词: 中华鳖  黄河  选育  微卫星  遗传变异
英文关键词: Chinese soft shelled turtle  Yellow River  selection  microsatellite  genetic variation
基金项目:浙江省绍兴市重点农业科研项目(2009A22027)
作者单位
张志允 上海海洋大学 农业部水产种质资源与利用重点开放实验室 
李思发 上海海洋大学 农业部水产种质资源与利用重点开放实验室 
蔡完其 上海海洋大学 农业部水产种质资源与利用重点开放实验室 
谢中富 绍兴国家级中华鳖原种场 
殷黎明 绍兴国家级中华鳖原种场 
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中文摘要:
      以中华鳖黄河群体的选育基础群体(F0)和太湖群体为对照群体,采用微卫星标记技术对黄河群体的选育世代F1、F2及F3的遗传变异进行了测定和分析。结果表明:(1)从所筛选的10个微卫星位点中,5个测试群体共检测出240个等位基因;平均等位基因数(Na) 为4.60~5.30,平均有效等位基因数(Ne)为3.24~3.59,平均观察杂合度(Ho)为0.483 3~0.530 0,平均期望杂合度(He)为0.594 9~0.645 9,平均多态信息含量(CPI)为0.534 0~0.568 4,这些遗传参数表明中华鳖存在丰富的可供选育用的遗传多样性基础。(2)F1、F2及F3选育世代的遗传多样性参数HoHeCPI一致地显示出随着选育代数的增加而降低的明显趋势,证明以生长、体色、体型等表型指标为直观选育指标的群体选育,对遗传型指标产生了可检测到的影响。(3)黄河群体的选育基础群体和3个选育世代在聚类分析中聚为一支,而太湖群体单独为一支,显示中华鳖的黄河、太湖两大地理群体间存在较大遗传分化,3代选育并未改变这一历史性格局。
英文摘要:
      To assess genetic variations during genetic selection of Chinese soft shelled turtle (Trionyx sinensis), the specimens of Chinese soft shelled turtle , collected from three selected generations ( F1,F2 and F3) of Yellow River population as testing group , and their base population (F0) as well as the Taihu population as control group, were amplified by ten pairs of microsatellite primers. The result showed that: (1)A total of 240 alleles were detected in all examined loci. In all five populations the value of average allele number (Na) were 4.60-5.30, the value of average of effective number of alleles (Ne) were 3.24-3.59; the value of average observed heterozygosity (Ho) were 0.483 3-0.530 0, the value of average expected heterozygosity (He) were 0.594 9-0.645 9 and the value of average polymorphism information content (CPI) were 0.534 0-0.568 4. (2)The three selected generation’s heredity multiple parameter Ho, He and CPI demonstrated decreased identically, along with the increases of selected generations. It indicated that the mass selection targeted on phenotypes such as growth, body color, body shape, produced a detectable effect on the genetype. (3)The phylogenetic tree showed that studied five populations were clustered into two groups, the first group consisted of the four Yellow River populations while the second group belongs to Taihu Lake population. It indicated that a significant genetic differentiation between Yellow River populations and Taihu Lake population existed and has not been changed by current three generations selection.
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