The variations of digestive enzyme activities during the development of Oplegnathus fasciatus larvae
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Key Words: Oplegnathus fasciatus  larvae  trypsinase  acid protease  amylase  lipase
Author NameAffiliation
YIN Yan-qiang 上海海洋大学 水产与生命学院 
FU Rong-bing 浙江省舟山市水产研究所 
HUANG Xu-xiong 上海海洋大学 水产与生命学院 
WEN Wen 上海海洋大学 水产与生命学院 
SHI Zhao-hong 中国水产科学研究院 东海水产研究所 
LUO Hai-zhong 浙江省舟山市水产研究所 
YAN Jia-qi 上海海洋大学 水产与生命学院 
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Abstract:
      This paper investigated the trypsinase, acid protease, amylase and lipase activities of the Oplegnathus fasciatus larvae cultured in indoor pond from hatching to post hatching day (phd) 33rd. The results indicated that trypsinase, acid protease, amylase and lipase could be detected in newly hatched larvae, although the activities of trypsinase, acid protease and amylase were very low. The total activity of trypsinase (U/larva) of the larvae increased significantly on phd 9th and increased gradually with the development. The total activities of acid prolease and lipase of the larvae increased significantly on phd 28th. The total activities of acid prolease and lipase of the larvae on phd 33rd were significant higher than those on phd 28th respectively. The total activity of amylase of the larvae increased significantly on phd 9th and also increased gradually with the development. While the larvae developed at juvenile stage (>phd 22nd), the total activity of amylase decreased significantly with the development. It is suggested that the activities of digestive enzyme of the Oplegnathus fasciatus larvae closely correspond to the development stage and feeding habit. The trypsinase and amylase were the very important endogenous enzymes at the early stage of the larva development.