Optimal extraction of inhibitory components from Myriophyllum spicatum with the ultrasonic-cellulase method
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Received:April 13, 2015  Revised:May 19, 2015
DOI:
Key Words: ultrasound-cellulase method  Myriophyllum spicatum  M. aeruginosa  growth inhibition rate
Author NameAffiliationE-mail
ZHAO Jintao College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China  
JIANG Min College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Research and Engineering Center on Aquatic Environment Ecosystem, Shanghai Ocean University, Shanghai 201306, China 
mjiang@shou.edu.cn 
WANG Junnan College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China  
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Abstract:
      The article explained that inhibitory components from Myriophyllum spicatum with the ultrasonic-cellulase method and water extraction method caused inhibition of M.aeruginosa. Based on one-way and orthogonal test, and regarding growth inhibition rate of extraction on M. aeruginosa as index of evaluation, an optimal extraction process of Myriophyllum spicatum was obtained as follows: enzymatic hydrolysis time was 4 h, pH was 3.0, optimal temperature was 30 ℃, cellulase dosage(mass of cellulase to Myriophyllum spicatum powder) was 21%, ultrasonic power was 120 W and ultrasonic time was 1h. The inhibition rate of extraction obtained with water extraction method and ultrasonic-cellulase method indicated that the latter showed better effect and had obvious difference with the water extraction (P<0.05). With the increasing of reaction time, the growth inhibition rate of the ultrasonic-cellulase extraction on M. aeruginosa continued to decrease, which was 271.74% at concentration of 40 g/L and reaction time for 24 h and the inhibition rate was more than 20% by the reaction time for 13d with the obvious difference with the other concentration groups (P<0.05).