Isolation and characterization of pigmentation mutants of Pyropia crispata(Bangiales, Rhodophyta)
View Fulltext  View/Add Comment  Download reader
Received:May 02, 2018  Revised:August 08, 2018
DOI:10.12024/jsou.20180502305
Key Words: Pyropia crispata  ultraviolet ray  color-mutated cell  pigmentation mutant  in vivo absorption spectrum
Author NameAffiliationE-mail
ZHANG Qian Key Laboratory of Exploration and Utilization of Aquatic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China 
 
DING Hongchang Key Laboratory of Exploration and Utilization of Aquatic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China 
 
YAN Xinghong Key Laboratory of Exploration and Utilization of Aquatic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China 
xhyan@shou.edu.cn 
LIU Changjun Xiangshan Fisheries Technical Extension Center, Xiangshan 315700, Zhejiang, China  
Hits: 126
Download times: 150
Abstract:
      The conchospore gametophytic blades of the wild-type strain of Pyropia crispata were irradiated with different doses of ultraviolet ray. After being cultured for about 4 weeks, many color-mutated cell clusters including yellow green, ruby red, yellow orange and so on,appeared on the irradiated blade. The statistical analysis showed most of the color-mutated cell types are yellow and the dose of 80 μW/cm2 attained the best mutagenic effect. Single mutated cell was isolated enzymatically from the color-mutated blades and was regenerated into blades, in which some single-color mutants were selected:PC-JH (orange brown), PC-ZH (ruby red), PC-HL (yellow green) and PC-QL (light green). Their homozygous strain were gotten by parthenogenesis, respectively. The color and morphology of the F1 gametophytic blades of each mutant strain showed uniform color and were same with its mother blade. The results indicated mutant characters such as color can be stably inherited in each mutant strain. Each mutant strain featured their own characteristics in in vivo absorption spectra of the blade, contents of chlorophyll a(Chl.a), phycocyanin(PC), phycoerythrin(PE) and their ratio, different from wild-type strain's. Compared with the wild-type strain (PC-WT), the content of PC and the value of PC/Chl.a of the two green-type strains(PC-HL, PC-QL) increased significantly, while the value of PE/PC decreased significantly and was close to one. The content of three pigment proteins in the orange-yellow mutant (PC-JH) was reduced, and the PC/Chl.a value was also lower, but the PE/Chl.a and PE/PC values were higher. The PE content of the date-red mutant (PC-ZH) increased significantly. Correspondingly, the values of PE/Chl.a and PE/PC also increased significantly. These results indicated that UV had a good mutagenic effect on Pyropia crispata blade, and the obtained four mutant strains were stable in color. They will become important materials for genetic breeding in Pyropia crispata.