Development and verification of SSR based on transcriptome of Sargassum horneri
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Received:June 29, 2018  Revised:January 07, 2019
DOI:10.12024/jsou.20180602354
Key Words: Sargassum horneri  transcriptome  SSR
Author NameAffiliationE-mail
LIU Ying Zhejiang Mariculture Research Institute, Wenzhou 325005, Zhejiang, China
Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Wenzhou 325005, Zhejiang, China 
 
ZHANG Peng Zhejiang Mariculture Research Institute, Wenzhou 325005, Zhejiang, China
Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Wenzhou 325005, Zhejiang, China 
 
WANG Tiegan Zhejiang Mariculture Research Institute, Wenzhou 325005, Zhejiang, China
Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Wenzhou 325005, Zhejiang, China 
wtg605@163.com 
ZHANG Min Zhejiang Mariculture Research Institute, Wenzhou 325005, Zhejiang, China
Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Wenzhou 325005, Zhejiang, China 
 
REN Peng Zhejiang Mariculture Research Institute, Wenzhou 325005, Zhejiang, China
Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Wenzhou 325005, Zhejiang, China 
 
ZHONG Chenhui Fisheries Research Institute of Fujian Province, Xiamen 361000, Fujian, China  
HU Chengrui Zhejiang Mariculture Research Institute, Wenzhou 325005, Zhejiang, China
Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Wenzhou 325005, Zhejiang, China 
 
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Abstract:
      SSRs were developed and characterized by using transcriptome data. A total of 2800 SSR loci were identified and dinucleotides were the most frequent (32.82%), followed by trinucleotides (25.21%). AC/GT was the most abundant unit (9.18%). The length of repetitive sequences was mainly concentrated between 12-20 bp (72.79%), and 5-10 repeats being the most abundant number (74.14%). 74 pairs of primers were randomly selected for detection, and 18 polymorphic SSRs were obtained finally. The characteristics of these microsatellites were determined in 31 individuals of S. horneri. A total of 43 alleles were detected, the allele number per locus ranged from 2 to 4 (average 2.39), and mean effective allele number was 1.91, and mean observed and expected heterozygosity were 0.318 and 0.449, respectively. The average of polymorphism information content (PIC) was 0.365, and 11 loci deviated significantly from Hardy-Weinberg equilibrium (HWE) (P<0.05). These obtained SSR markers will be helpful for the genetic studies in S. horneri.