Effects of different cultural patterns on microbial communities in the intestine of Macrobrachium rosenbergii and interactions with environment factors
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Received:September 10, 2018  Revised:December 25, 2018
DOI:10.12024/jsou.20180902396
Key Words: Macrobrachium rosenbergii  cultural pattern  intestine  bacterial community  environmental factor  interaction
Author NameAffiliationE-mail
DONG Xuexing Key Laboratory for Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng 224051, Jiangsu, China
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China 
 
LÜ Linlan Key Laboratory for Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng 224051, Jiangsu, China Lvlinlan@126.com 
ZHAO Weihong Key Laboratory for Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng 224051, Jiangsu, China  
YU Yebing Key Laboratory for Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng 224051, Jiangsu, China  
LIU Qigen College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China  
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Abstract:
      Microbial communities in the intestine of Macrobrachium rosenbergii under six cultural patterns were evaluated by high-throughput pyrosequencing technology. The interactions between microbial and water environment were also analyzed by RDA (Redundancy analysis). The cultural patterns included prawn monoculture group (MP), prawn with aquatic plant (Lemna minor) group (PP), prawn with silver carp (Hypophthalmichthys molitrix) group (PF), prawn with mussel (Anodonta woodiana) and H. molitrix group (PMF), prawn with mussel and aquatic plant group (PMP), prawn with A. woodiana, L. minor and H. molitrix group (PMPF). Four replicates of each treatment were set up. After 64 days of culturing, the microbial communities and physico-chemical factors were assessed. There were significant differences in turbidity, soluble phosphorus (PO4-P), total nitrogen (TN) and chlorophyll-a (Chl.a) among groups, and PO4-P concentration in MP group was significantly higher than those in other groups (P<0.05). Shannon diversity index of intestinal bacteria was the highest in the MP group (4.08), and the lowest in the PMF group (1.27). Proteobacteria, Tenericute and Firmicutes were the major dominant phyla in intestine of M. rosenbergii in the six groups. The major dominant genus was different in six groups, which was Aeromonas in MP, PMP and PMPF groups, and Citrobacter in PP group, and Candidatus and Hepatoplasma in PF and PMF groups. The interaction analysis between intestinal bacteria and environmental factors showed that total phosphorus (TP) had significant effects on the intestinal bacterial community of M. rosenbergii (P<0.05), Adaptation of microorganisms to the environment factors was different, and the abundance of Aeromonas enteropelogenes, beneficial Enterococcus and Lactococcus garvieae were positive correlation with NO3-N and TN. Rhodobacter and Pseudomonas vranovensis were positive correlation with TP. Conclusively, cultural patterns can affect the intestinal bacterial community of M. rosenbergii by affecting the nutrient concentration in the water, especially nitrogen and phosphorus.