文章摘要
丁红兵,李浩宇,陈义华,陈丽芝,刘琦,牛东红.高盐对缢蛏生长存活、Na+/K+-ATPase活性及能量代谢相关指标的影响[J].上海海洋大学学报,2022,31(4):831-838.
DING Hongbing,LI Haoyu,CHEN Yihua,CHEN Lizhi,LIU Qi,NIU Donghong.Effects of high salinity on growth and survival, Na+/K+-ATPase activity and energy metabolism related indexes of razor clam Sinonovacula constricta[J].Journal of Shanghai Ocean University,2022,31(4):831-838.
高盐对缢蛏生长存活、Na+/K+-ATPase活性及能量代谢相关指标的影响
Effects of high salinity on growth and survival, Na+/K+-ATPase activity and energy metabolism related indexes of razor clam Sinonovacula constricta
投稿时间:2021-03-28  修订日期:2021-07-27
DOI:10.12024/jsou.20210303356
中文关键词: 缢蛏  高盐  生长  存活率  Na+/K+-ATPase活性  能量代谢
英文关键词: Sinonovacula constricta  high salinity  growth  survival rate  Na+/K+-ATPase activity  energy metabolism
基金项目:国家重点研发计划(2019YFD0900700);国家自然科学基金(31472278)
作者单位E-mail
丁红兵 上海海洋大学 水产种质资源发掘与利用教育部重点实验室, 上海 201306  
李浩宇 上海海洋大学 水产种质资源发掘与利用教育部重点实验室, 上海 201306  
陈义华 上海海洋大学 水产种质资源发掘与利用教育部重点实验室, 上海 201306  
陈丽芝 三门县农业农村局, 浙江 台州 317100  
刘琦 三门县农业农村局, 浙江 台州 317100  
牛东红 上海海洋大学 水产种质资源发掘与利用教育部重点实验室, 上海 201306
上海海洋大学 水产动物遗传育种中心上海市协同创新中心, 上海 201306 
dhniu@shou.edu.cn 
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中文摘要:
      以缢蛏(Sinonovacula constricta)为研究对象,设置养殖周期为60 d的对照组(20)和高盐组(30)进行比较实验,分别在0、10、30和60 d取样,检测高盐对缢蛏生长存活、鳃组织Na+/K+-ATPase (NKA)活性及肝胰腺和血清能量代谢相关指标的影响。结果表明,高盐组缢蛏在30~60 d的养殖时间段内存活率为45.1%±2.9%,壳长、壳宽、壳高和体质量特定生长率分别为0.15%/d、0.17%/d、0.13%/d和0.61%/d,均显著低于对照组。各时间点高盐组NKA活性均显著低于对照组。10和60 d时对照组缢蛏肝胰腺己糖激酶(HK)活性显著高于高盐组,血清中葡萄糖(GLU)含量、乳酸(LA)含量和乳酸脱氢酶(LDH)活性也显著高于高盐组,高盐组缢蛏无氧代谢水平低于对照组。30 d时高盐组血清中GLU含量、LA含量和LDH活性显著高于对照组,高盐组缢蛏无氧代谢水平高于对照组。0、10和30 d时高盐组肝胰腺琥珀酸脱氢酶(SDH)活性显著高于对照组,高盐胁迫下缢蛏需氧代谢水平上升。研究表明:长期高盐养殖下,缢蛏仍能保持一定的存活率和生长速度;高盐影响了缢蛏的渗透调节和能量代谢,渗透调节增加了其能量消耗。本研究为进一步培育缢蛏耐高盐新品系提供了参考资料。
英文摘要:
      To understand the effects of high-salinity on razor clam Sinonovacula constricta, the razor clams were cultured at salinity of 20 (control group) and 30 (experimental group) for 60 days. The growth and survival, Na+/K+-ATPase (NKA) activity in gills, and indexes of energy metabolism in hepatopancreas and serum were measured at the time point of 0, 10, 30, and 60 d. The results showed that the survival rate of razor clam cultured in experimental group was 45.1%±2.9% in 30-60 d. The specific growth rate of shell length, shell width, shell height and body weight of razor clam cultured in experimental group were 0.15%/d, 0.17%/d, 0.13%/d and 0.61%/d respectively in 60 days, and all of these indexes were significantly lower than those in the control group. The NKA activity of experimental group was significantly lower than control group at the time point of 0, 10, 30, and 60 d. The activity of hexokinase (HK) in hepatopancreas of control group was significantly higher than experimental group at the time point of 10 and 60 d, the same with glucose (GLU), lactic acid (LA) content and lactate dehydrogenase (LDH) activity in serum, which indicated that the anaerobic metabolism level of experimental group was lower than control group at those two time points. The GLU, LA content and LDH activity in serum of experimental group were significantly higher than those in the control group at the time point 30 d, which indicated that the anaerobic metabolism level of experimental group was higher than control group at the time point 30 d. The succinate dehydrogenase (SDH) activity in hepatopancreas of experimental group was significantly higher than control group at the time point 0, 10 and 30 d, which indicated that the level of aerobic metabolism of razor clam increased under high salt stress. In summary, razor clam can maintain a certain survival rate and a certain growth rate under long-term high-salt culture. The osmotic adjustment and the energy metabolism of razor clam have been affected under high-salt culture, and the osmotic adjustment increased its energy consumption. This study provides reference materials for further cultivation of new high-salt-tolerant strains of razor clam.
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