文章摘要
郭思鹏,汪桂玲,白志毅,孙田洋.雌性三角帆蚌外鳃组织形态周年变化及低氧胁迫对外鳃组织形态和酶活性影响[J].上海海洋大学学报,2022,31(4):865-872.
GUO Sipeng,WANG Guiling,BAI Zhiyi,SUN Tianyang.Annual changes of outer gill tissue morphology and effects of hypoxia stress on outer gill tissue morphology and enzyme activities in female Hyriopsis cumingii[J].Journal of Shanghai Ocean University,2022,31(4):865-872.
雌性三角帆蚌外鳃组织形态周年变化及低氧胁迫对外鳃组织形态和酶活性影响
Annual changes of outer gill tissue morphology and effects of hypoxia stress on outer gill tissue morphology and enzyme activities in female Hyriopsis cumingii
投稿时间:2020-04-10  修订日期:2021-06-22
DOI:10.12024/jsou.20210403386
中文关键词: 三角帆蚌  外鳃  育儿囊  低氧  酶活性
英文关键词: Hyriopsis cumingii  outer gill  nursery sac  hypoxia  enzyme activity
基金项目:国家重点研发计划(2018YFD0901406);现代农业产业技术体系建设专项(CARS-49);江苏省苏北科技专项(SZ-SQ2019045)
作者单位E-mail
郭思鹏 上海海洋大学 农业农村部淡水水产种质资源重点实验室, 上海 201306
上海海洋大学 水产动物遗传育种中心上海市协同创新中心, 上海 201306 
 
汪桂玲 上海海洋大学 农业农村部淡水水产种质资源重点实验室, 上海 201306
上海海洋大学 水产动物遗传育种中心上海市协同创新中心, 上海 201306
上海海洋大学 上海水产养殖工程技术研究中心, 上海 201306 
 
白志毅 上海海洋大学 农业农村部淡水水产种质资源重点实验室, 上海 201306
上海海洋大学 水产动物遗传育种中心上海市协同创新中心, 上海 201306
上海海洋大学 上海水产养殖工程技术研究中心, 上海 201306 
zybai@shou.edu.cn 
孙田洋 上海海洋大学 农业农村部淡水水产种质资源重点实验室, 上海 201306
上海海洋大学 上海水产养殖工程技术研究中心, 上海 201306 
 
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中文摘要:
      通过石蜡切片观察雌性三角帆蚌外鳃组织形态周年变化,并探究低氧环境对雌性三角帆蚌外鳃组织形态及酶活性的影响。结果表明,雌性三角帆蚌外鳃鳃瓣1月最薄,至5月增至最厚,较1月显著增厚173.86%,随后鳃瓣逐渐变薄,其鳃小腔宽度随鳃瓣厚度增厚也显著增大,至6月增至最宽较1月显著增宽171.22%,随后鳃小腔逐渐变窄,且3—6月在雌性三角帆蚌外鳃发育为育儿囊,其中发现大量早期胚胎。在低氧环境下,乳酸脱氢酶(LDH)活性在1~5 d逐渐升高,第5天达到最大值,较对照组显著提升131.1%,5~9 d缓慢下降;超氧化物歧化酶(SOD)活性在1~7 d逐渐下降,在第7天达到最小值,较对照组显著降低40.53%,7~9 d缓慢上升;过氧化氢酶(CAT)活性在1~7 d逐渐上升,7~9 d无明显变化,第7天活性最高,较对照组显著提升91.34%,琥珀酸脱氢酶(SDH)活性在1~9 d呈下降趋势,最低值出现在第9天,较对照组显著降低68.9%;低氧胁迫5 d后,外鳃侧纤毛较对照组增宽了79.58%,外鳃小水管较对照组增宽了248.73%。本研究分别为怀卵期雌性三角帆蚌外鳃组织结构变化和低溶氧对雌性三角帆蚌外鳃组织结构和酶活性的影响提供了基础资料,为提升三角帆蚌繁育技术提供了理论依据。
英文摘要:
      The outer gill of female Hyriopsis cumingii is not only a respiratory organ, but also performs the function of embryo sac during the reproduction period. The respiratory efficiency of female Hyriopsis cumingii was decreased during the breeding period. The annual changes of the outer gill tissue morphology of female Hyriopsis cumingii were observed by paraffin section, and the effects of hypoxia environment on the outer gill tissue morphology and enzyme activities of female Hyriopsis cumingii were explored. The results showed that the outer gill of female Hyriopsis cumingii was the thinnest in January, and increased to the thickest in May, which was 173.86% thicker than that in January. The gill gradually became thinner, and the width of the gill cavity also increased significantly with the thickness of the gill, and increased to the widest in June, which was 171.22% wider than that in January, and the gill cavity gradually narrowed there were a large number of early embryos. In hypoxia environment, the activity of lactate dehydrogenase (LDH) increased gradually in 1-5 days, reached the maximum value on the 5th day, increased by 131.1% compared with the control group, and decreased slowly in 5-9 days; The activity of superoxide dismutase (SOD) decreased gradually in 1-7 days, reached the minimum value on the 7th day, decreased by 40.53% compared with the control group, and increased slowly in 7-9 days; The activity of catalase (CAT) increased gradually in 1-7 days. The activity of succinate dehydrogenase (SDH) decreased from 1 to 9 days, and the lowest value appeared on the 9th day, which was 68.9% lower than that of the control group. After 5 days of hypoxia stress, the cilia on the lateral side of the outer gill were 79.58% wider than that of the control group, and the aqueduct of the outer gill were 248.73% wider than that of the control group. This study provided basic data for the changes of outer gill tissue structure of female Hyriopsis cumingii and the effects of low dissolved oxygen on the tissue structure and enzyme activities of outer gill of female Hyriopsis cumingii, and provided theoretical basis for improving the breeding technology of Hyriopsis cumingii.
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