文章摘要
罗兴,毕清竹,孟晓雪,刘晋士,卫育良,段美,徐后国,梁萌青.高脂饲料中添加含硫氨基酸对大菱鲆脂肪代谢的影响[J].上海海洋大学学报,2022,31(4):893-905.
LUO Xing,BI Qingzhu,MENG Xiaoxue,LIU Jinshi,WEI Yuliang,DUAN Mei,XU Houguo,LIANG Mengqing.Effects of supplementation of sulfur-containing amino acids in high-lipid diets on lipid metabolism of turbot (Scophthalmus maximus)[J].Journal of Shanghai Ocean University,2022,31(4):893-905.
高脂饲料中添加含硫氨基酸对大菱鲆脂肪代谢的影响
Effects of supplementation of sulfur-containing amino acids in high-lipid diets on lipid metabolism of turbot (Scophthalmus maximus)
投稿时间:2021-12-28  修订日期:2022-03-04
DOI:10.12024/jsou.20211203660
中文关键词: 大菱鲆  蛋氨酸  半胱氨酸  牛磺酸  脂代谢
英文关键词: Scophthalmus maximus  methionine  cysteine  taurine  lipid metabolism
基金项目:中国水产科学研究院基本科研业务费项目(2020TD48);农业农村部和财政部现代农业产业技术体系项目(CARS-47)
作者单位E-mail
罗兴 大连海洋大学 水产与生命学院, 辽宁 大连 116023
中国水产科学研究院黄海水产研究所, 山东 青岛 266071 
 
毕清竹 中国水产科学研究院黄海水产研究所, 山东 青岛 266071  
孟晓雪 中国水产科学研究院黄海水产研究所, 山东 青岛 266071  
刘晋士 中国水产科学研究院黄海水产研究所, 山东 青岛 266071  
卫育良 中国水产科学研究院黄海水产研究所, 山东 青岛 266071
青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266071 
 
段美 中国水产科学研究院黄海水产研究所, 山东 青岛 266071  
徐后国 中国水产科学研究院黄海水产研究所, 山东 青岛 266071
青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266071 
 
梁萌青 中国水产科学研究院黄海水产研究所, 山东 青岛 266071
青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266071 
liangmq@ysfri.ac.cn 
摘要点击次数: 2241
全文下载次数: 1348
中文摘要:
      为了探究高脂饲料中添加蛋氨酸、半胱氨酸及牛磺酸对大菱鲆(Scophthalmus maximus)脂肪代谢的影响,在高脂(16%)基础饲料(对照组)中分别添加1.5%的蛋氨酸、半胱氨酸和牛磺酸制成4种等氮等脂饲料。试验选用平均初始体质量为(7.98±0.02) g的大菱鲆幼鱼为研究对象,每组饲料投喂3个重复桶,每桶30尾鱼,在室内流水系统中进行为期56 d的摄食生长试验。结果表明:全鱼和肌肉粗脂肪在各组间没有显著差异。肝脏粗脂肪含量在牛磺酸组最低,显著低于半胱氨酸组。血清甘油三酯和总胆固醇含量在牛磺酸组最高,显著高于其他组。饲料中含硫氨基酸的添加降低了鱼体EPA含量,牛磺酸升高了鱼体C18∶2n-6和C18∶3n-3含量。饲料中含硫氨基酸的添加显著影响了大菱鲆肝脏脂肪代谢基因表达,蛋氨酸升高了脂肪酸从头合成相关基因(FAS)的表达;半胱氨酸升高了甘油酯生成相关基因DGAT1的表达,而牛磺酸降低了脂肪合成相关基因(ACACβ、FAS、SREBP1、PPARα1、PPARγLXRα)的表达。生长方面,终末体质量在牛磺酸组显著高于其他各组,但各处理组间成活率、肥满度、肝体比和脏体比均无显著差异。在大菱鲆高脂饲料中添加蛋氨酸、半胱氨酸及牛磺酸对大菱鲆脂肪代谢具有调节作用,牛磺酸具有降低肝脏脂肪含量的作用,而半胱氨酸具有升高肝脏脂肪含量的作用。
英文摘要:
      A 56-day feeding trail was conducted to investigate the effects of methionine, cysteine and taurine on lipid accumulation and lipid metabolism of turbot (Scophthalmus maximus) fed high levels of dietary lipid. A high-lipid (16%) diet was used as the control diet, and 1.5% methionine (Met), 1.5% cysteine (Cys), and 1.5% taurine (Tau) was separately added into the control diets to obtain three test diets. Fish with average initial weight of (7.98±0.02) g was used. Each diet was fed to triplicate tanks of 30 fish. The feeding trial was conducted in flow-through seawater system. The results showed that no significant differences were observed among treatments in lipid content of whole body and muscle. Group Tau had the lowest liver lipid content, significantly lower than group Cys. The serum triacylglycerol and total cholesterol contents were the highest in group Tau, significantly higher than other groups. The dietary supplementation of sulfur-containing amino acids decreased the EPA content in fish body, while Tau increased the contents of C18:2n-6 and C18:3n-3. The dietary supplementation of sulfur-containing amino acids significantly regulated the lipid metabolism-related gene expression. Dietary Met supplementation up-regulated the liver mRNA expression of FAS; Dietary Cys supplementation up-regulated the liver mRNA expression of DGAT1; Dietary Tau supplementation down-regulated the hepatic mRNA expression of a series of lipogenic genes such as ACACβ, FAS, SREBP1, PPARα1, PPARγ and LXRα. Regarding the growth performance, group Tau had significantly higher final body weight of fish than other groups, but no significant difference was observed in survival, condition factor, hepatosomatic index and viscerosomatic index among dietary groups. Supplementation of methionine, cysteine and taurine in high-lipid diets regulates the lipid metabolism of turbot. Taurine appears to be able to reduce the hepatic lipid accumulation in turbot but cysteine tends to increase it.
HTML 查看全文   
关闭