文章摘要
徐先栋,付辉云,饶毅,盛银平,王海华,傅义龙,李彩刚,周永灿.草鱼暴发性出血病病原分离、毒力基因检测与药敏分析[J].上海海洋大学学报,2016,25(3):350-358.
XU Xiandong,FU Huiyun,RAO Yi,SHENG Yinping,WANG Haihua,FU Yilong,LI Caigang,ZHOU Yongcan.The virulence characteristics and drug susceptibility of outbreak hemorrhage disease pathogen isolated from grass carp Ctenopharyngodon idllus[J].Journal of Shanghai Ocean University,2016,25(3):350-358.
草鱼暴发性出血病病原分离、毒力基因检测与药敏分析
The virulence characteristics and drug susceptibility of outbreak hemorrhage disease pathogen isolated from grass carp Ctenopharyngodon idllus
投稿时间:2015-06-18  修订日期:2015-09-25
DOI:10.12024/jsou.20150601483
中文关键词: 嗜水气单胞菌  ERIC-PCR分型  毒力因子  药敏试验
英文关键词: Aeromonas hydrophila  ERIC-PCR  virulence gene  drug susceptibility test
基金项目:国家科技支撑计划项目(2012BAD25B07);海南省重点科技计划项目(ZDXM20120005);江西省发明专利产业化技术示范项目(20143BBM26038);江西省水产科学研究所青年基金项目(SC201302)
作者单位E-mail
徐先栋 海南大学 海洋学院, 海南 海口 570228
江西省水产科学研究所, 江西 南昌 330039 
 
付辉云 江西省水产科学研究所, 江西 南昌 330039  
饶毅 江西省水产科学研究所, 江西 南昌 330039  
盛银平 江西省水产科学研究所, 江西 南昌 330039  
王海华 江西省水产科学研究所, 江西 南昌 330039  
傅义龙 江西省水产科学研究所, 江西 南昌 330039  
李彩刚 江西省水产科学研究所, 江西 南昌 330039  
周永灿 海南大学 海洋学院, 海南 海口 570228 zychnu@163.com 
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中文摘要:
      从草鱼体内分离到致病菌株Jxsks1,该菌株对草鱼的LD50值为2.3×103 CFU/g鱼体质量,为强致病菌;经生理生化、16S rDNA和gyrB基因序列测序鉴定为嗜水气单胞菌。ERIC-PCR分型结果表明,该菌株为迄今未见报道的类型。选取丝氨酸蛋白酶ahp、热稳定性肠毒素ast、气溶素aerA、热不稳定性肠毒素alt、鞭毛基因fla、脂酶lip等6种毒力基因特异性引物对该菌株进行PCR检测,结果表明其毒力基因型为aerA+alt+ast+ahp+lip+fla-。选取28种抗生素进行药敏检测结果表明,该菌株对喹诺酮类、氨基糖苷类、四环素类、氯霉素、第二代和第三代头孢类等药物敏感,对硝基呋喃类、大环内酯类、多肽类中度敏感,对第一代头孢类、青霉素类、复方新诺明和洁霉素耐药。
英文摘要:
      A strong pathogenic bacterial strain Jxsks1 was isolated from grass carp, Ctenopharyngodon idllus. The pathogen was identified as Aeromonas hydrophila based on the physiological and biochemical analysis and the sequencing of housekeeping gene 16S rDNA and gyrB. The LD50 of the bacteria infecting the grass carp via intraperitoneal injection was 2.3×103 CFU/g. The results of genetic typing by ERIC-PCR (enterobacterial repetitive intergenic consensus PCR)-fingerprinting showed that it was a type of A. hydrophila which has not been reported before. The distribution patterns of six virulence genes, including aerolysin (aerA), heat-labile cytotonic enterotoxin (alt), heat-stable cytotoxic enterotoxin (ast), elastase (ahpB), lipase (lip) and flagella (fla), were tested by PCR method and the results showed that its virulence-gene pattern was aerA+, alt+, ast+, ahp+, lip+, fla-. The drug susceptibility test of 28 antibiotics by paper extension method showed that the strain was susceptible to quinolones, aminoglycoside, tetracyclines, chloramphenicol, the second and the third generation cephalosporins, intermediately sensitive to nitrofurans, macrolides and polypeptides, but resistant to the first generation cephalosporins, penicillins, sulfamethoxazole compound and lincomycin.
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