文章摘要
付娇娇,王旭,刘海泉,孙晓红,谢晶,潘迎捷,赵勇.不同培养条件下sigB对单增李斯特菌生物被膜形成的影响[J].上海海洋大学学报,2016,25(4):634-640.
FU Jiaojiao,WANG Xu,LIU Haiquan,SUN Xiaohong,XIE Jing,PAN Yingjie,ZHAO Yong.Effects of sigB on biofilm formation by Listeria monocytogenes under various culture conditions[J].Journal of Shanghai Ocean University,2016,25(4):634-640.
不同培养条件下sigB对单增李斯特菌生物被膜形成的影响
Effects of sigB on biofilm formation by Listeria monocytogenes under various culture conditions
投稿时间:2015-12-07  修订日期:2016-02-27
DOI:10.12024/jsou.20151201610
中文关键词: 单增李斯特菌  生物被膜  sigB
英文关键词: Listeria monocytgenes  biofilm  sigB
基金项目:国家自然科学基金面上项目(31271870,31571917);国家科技支撑计划(2015BAD17B01);上海市科委计划项目(14DZ1205100,14320502100);上海市科技兴农重点攻关项目(沪农科攻字2014第3-5号、2015第4-8号);上海水产品加工及贮藏工程技术研究中心(11DZ2280300)
作者单位E-mail
付娇娇 上海海洋大学 食品学院, 上海 201306  
王旭 上海海洋大学 食品学院, 上海 201306  
刘海泉 上海海洋大学 食品学院, 上海 201306  
孙晓红 上海海洋大学 食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海 201306
农业部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306 
 
谢晶 上海海洋大学 食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海 201306
农业部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306 
 
潘迎捷 上海海洋大学 食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海 201306
农业部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306 
 
赵勇 上海海洋大学 食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海 201306
农业部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306 
yzhao@shou.edu.cn 
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中文摘要:
      本研究比较分析了不同温度(4、15、25和37℃)、pH(4、5、6和7)及NaCl浓度(0.5%、2.5%、4.5%和6.5%)对单增李斯特菌野生型菌株(WaX12)及sigB缺失突变型菌株(WaX12sigB)生物被膜形成能力的影响。结果表明,相比于WaX12菌株,WaX12sigB菌株生物被膜的形成量显著降低(P<0.05)。变异系数分析显示,不同培养条件对WaX12菌株及WaX12sigB菌株生物被膜形成能力均有影响,其中温度的影响最大,NaCl浓度次之,pH最弱;且WaX12sigB菌株生物被膜形成能力更易受到培养条件的影响。其次,分别选取了WaX12菌株与WaX12sigB菌株生物被膜形成量差异最显著的培养条件(37℃、pH 6及2.5% NaCl)进行后续分析。结果发现WaX12sigB菌株胞外多糖及胞外蛋白的相对含量均显著降低(P<0.05),而其活菌数略有降低。本研究为深入探讨sigB参与单增李斯特菌生物被膜形成的分子机制提供科学依据。
英文摘要:
      The role of sigB, a major transcriptional regulator of stress response genes of Listeria monocytogenes, was assessed in biofilm formation of wild type strain (WaX12) and sigB deletion mutant (WaX12sigB) as affected by temperatures (4℃, 15℃, 25℃ and 37℃), pH values (4, 5, 6 and 7) and NaCl concentrations (0.5%, 2.5%, 4.5% and 6.5%). Results showed that the biofilm biomass was significantly reduced in L. monocytogenes mutant lacking sigB (P<0.05). According to the coefficient of variation analysis, all three of the culture conditions tested (i.e., temperature, pH and NaCl concentration) appeared to affect the strain variability of the biofilm formation of WaX12 and WaX12sigB, and the increase in the strain variability of biofilm formation caused by temperature is much greater than that caused by NaCl or pH. The ability of forming biofilm by WaX12sigB was more vulnerable to the effects of external culture conditions. There was a significant difference of biofilm biomass between WaX12 and WaX12sigB at 37℃, pH 6 and 2.5% NaCl, respectively, so the further research was observed under these culture conditions. Notably, results showed that the relative amounts of total polysaccharides and extracellular proteins were much lower in WaX12sigB biofilm (P<0.05). However, there was little influence on live cells. In conclusion, this study could provide a scientific basis for further investigation of the important role of sigB in L. monocytogenes biofilm formation.
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