文章摘要
何强,常玉梅,苏宝锋,孙博,孙效文,梁利群.碳酸盐碱度对达里湖瓦氏雅罗鱼耗氧率、氨氮排泄和排氨基因表达的影响[J].上海海洋大学学报,2016,25(4):551-558.
HE Qiang,CHANG Yumei,SU Baofeng,SUN Bo,SUN Xiaowen,LIANG Liqun.Effects of carbonate alkalinities on oxygen consumption, ammonia excretion and ammonia excretion gene expression in Leuciscus waleckii Dybowski[J].Journal of Shanghai Ocean University,2016,25(4):551-558.
碳酸盐碱度对达里湖瓦氏雅罗鱼耗氧率、氨氮排泄和排氨基因表达的影响
Effects of carbonate alkalinities on oxygen consumption, ammonia excretion and ammonia excretion gene expression in Leuciscus waleckii Dybowski
投稿时间:2016-02-21  修订日期:2016-03-16
DOI:10.12024/jsou.20160201663
中文关键词: 达里湖瓦氏雅罗鱼  碳酸盐碱度  耗氧率  排氨率  血氨含量  Rh基因  氧氮比
英文关键词: Leuciscus waleckii Dybowski  carbonate alkalinity  oxygen consumption rate  ammonia excretion rate  plasma ammonia content  Rh genes  oxygen-nitrogen ratio
基金项目:国家自然科学基金(31461163004);黑龙江省科技攻关项目(GCI2B307)
作者单位E-mail
何强 中国水产科学研究院黑龙江水产研究所 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070
上海海洋大学 水产与生命学院, 上海 201306 
 
常玉梅 中国水产科学研究院黑龙江水产研究所 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070  
苏宝锋 中国水产科学研究院黑龙江水产研究所 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070  
孙博 中国水产科学研究院黑龙江水产研究所 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070  
孙效文 中国水产科学研究院黑龙江水产研究所 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070  
梁利群 中国水产科学研究院黑龙江水产研究所 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070 llq-1019@163.com 
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中文摘要:
      采用封闭流水式呼吸室法,研究了不同浓度碳酸盐碱度(以下简称CA, 10、30、50、70、90、110 mmol/L)对体质量为(84.62±6.70)g的达里湖瓦氏雅罗鱼耗氧率、氨氮排泄率、血氨含量及排氨基因Rh家族(RhagRhbgRhcg1Rhcg2)在鳃组织中表达量的影响。结果表明,随着碱度的升高,瓦氏雅罗鱼的耗氧率和排氨率均表现为先下降后上升的趋势,且都显著低于对照组(P<0.05)。在CA90时,耗氧率和排氨率均达到最低值,表明CA90可能是达里湖瓦氏雅罗鱼所能承受的最大碱度胁迫。相反,随着碱度的升高,瓦氏雅罗鱼的血氨含量呈现先上升后下降的趋势,在CA90时达到峰值。然而在CA0~50时,瓦氏雅罗鱼血氨含量无显著差异(P>0.05),表明达里湖瓦氏雅罗鱼进化出特殊的排氨机制,使其体内能够保持较低的氨氮含量以此规避氨中毒。定量PCR显示,参与氨转运的4种Rh基因在不同碱度处理组均有不同程度的上调表达;其中Rhcg1Rhcg2在高碱度(CA110)最为显著(P<0.05),表明Rhcg1Rhcg2可能在达里湖瓦氏雅罗鱼高碱胁迫下的排氨过程中发挥重要作用。能量代谢研究(O:N)发现,在CA0~70时,雅罗鱼无需消耗过多能源物质即可实现高碱度胁迫下的能量需求;而在碱度为90~110时,则主要消耗脂肪和碳水化合物供能,减少蛋白质耗能,以此降低毒氨的产生。依据上述研究结果,深入探讨了达里湖瓦氏雅罗鱼在高碱胁迫下的排氨策略及其可能的生理和分子机制,为推动达里湖瓦氏雅罗鱼在不同类型盐碱水域的增养殖提供科学依据。
英文摘要:
      Effects of different concentrations of carbonate alkalinities on oxygen consumption rate, ammonia excretion rate, plasma ammonia content as well as relative gene expression profile of Rh family in gill of Leuciscus waleckii Dybowski fish were studied using closed water flow respiration measurement chamber. Oxygen consumption rate and ammonia excretion rate were significantly inhibited in different alkalinity conditions in comparison with those in control, and the lowest values of them were found at CA90, which indicated CA90 may be the alkalinity limit for this species. Contrarily, the plasma ammonia content showed the opposite trend, first increasing, then decreasing, and peaked at CA90. No significant difference was found for plasma ammonia content when CA ranged from 0 to 50 mm, indicating this species has evolved to have specific ammonia excretion mechanism and thus helps to maintain the low level of toxic ammonia form. Quantitative real-time PCR showed that Rhcg1 and Rhcg2 out of the four tested genes in Rh family may play an important role in the process of ammonia excretion under high alkaline stress. Energy metabolism (O:N) study found that L. waleckii Dybowski did not consume too much energy to satisfy the energy needs within tolerance range (CA0-70); however, when the environmental alkalinity was between 90 and 110, the body fat and carbohydrates became the main energy sources to reduce protein consumption, which in turn decreased the poisonous ammonia excretion. This study served to elucidate the ammonia excretion strategy and possible physiological and molecular mechanism of ammonia excretion in L. waleckii Dybowski under high alkaline stress and it may provide evidence for aquaculturing L. waleckii Dybowski species in different carbonate alkaline water bodies.
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