文章摘要
莘似韵,孙晓红,徐忆宁,赵勇,吴启华,潘迎捷.超高压对单增李斯特菌生物被膜形成的影响[J].上海海洋大学学报,2017,26(2):294-300.
SHEN Siyun,SUN Xiaohong,XU Yining,ZHAO Yong,WU Vivian Chihua,PAN Yingjie.Effects of ultra-high pressure (UHP) processing on biofilm formation of Listeria monocytogenes[J].Journal of Shanghai Ocean University,2017,26(2):294-300.
超高压对单增李斯特菌生物被膜形成的影响
Effects of ultra-high pressure (UHP) processing on biofilm formation of Listeria monocytogenes
投稿时间:2016-04-11  修订日期:2016-07-22
DOI:10.12024/jsou.20160401731
中文关键词: 超高压  单增李斯特菌  生物被膜  SEM
英文关键词: UHP processing  Listeria monocytogenes  biofilm  SEM
基金项目:上海市科技兴农重点攻关项目(沪农科攻字2014第3-5号);上海市科委工程中心能力提升项目(16DZ22803001)
作者单位E-mail
莘似韵 上海海洋大学 食品学院, 上海 201306  
孙晓红 上海海洋大学 食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心农业部, 上海 201306
水产品贮藏保鲜质量安全风险评估实验室, 上海 201306 
xhsun@shou.edu.cn 
徐忆宁 上海海洋大学 食品学院, 上海 201306  
赵勇 上海海洋大学 食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心农业部, 上海 201306
水产品贮藏保鲜质量安全风险评估实验室, 上海 201306 
 
吴启华 美国USDA-ARS研究中心, 美国加利福尼亚州 94710  
潘迎捷 上海建桥学院, 上海 201306  
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中文摘要:
      为探究超高压处理对单增李斯特菌被膜形成能力的影响。以2株4b血清型的单增李斯特菌(WaX12、ATCC13932)为研究对象进行超高压处理(100~500 MPa,15 min,20℃),通过24孔板结晶紫染色方法对2株菌生物被膜形成能力进行检测,结合荧光显微镜和扫描电镜来观察生物被膜形成情况。WaX12比ATCC13932形成被膜的能力强。100 MPa压力处理显著增强WaX12的被膜形成能力(P<0.05),200 MPa压力处理使ATCC13932被膜形成量显著增多(P<0.05),而大于300 MPa的超高压处理使2株菌的被膜形成量均显著减少(P<0.05)。荧光显微镜和扫描电镜观察结果与结晶紫染色结果相符,超高压处理对2株单增李斯特菌被膜形成能力均有影响。小于200 MPa压力处理后单增李斯特菌被膜形成能力增强,并在48 h后被膜形成量达到最大值。
英文摘要:
      We found Listeria monocytogenes was still alive after the ultra-high pressure processing. Based on the result of our former experiments, this research aims to investigate whether the ultra-high pressure processing affects the biofilm formation of L. monocytogenes. 2 strains of L. monocytogenes were applied in the research, both serotypes were 4b.Crystal violet staining assay was used to investigate the biofilm forming capacity of 2 strains, while the fluorescent microscopy and SEM were applied to observe the structures of the biofilm. The biofilm forming capacity of WaX12 was higher than ATCC13932. After 100 MPa UHP processing, WaX12 formed more biofilm than the control group, while 200 MPa improved the biofilm forming capacity of ATCC13932. The biofilm formation of both strains reduced after 300 MPa and higher UHP processing. The images of biofilm formation of the 2 strains obtained by fluorescent microscopy and SEM were consistent with the results of crystal violet staining assay. Results suggested that the UHP processing may increase the biofilm formation of L. monocytogenes. If the pressure was lower than 200 MPa, the biofilm formation was strengthened, and reached the maximum after 48 h. It is important to choose proper pressure during UHP processing.
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