文章摘要
张娜,韦芬,鲍宝龙.星点东方鲀及共生细菌河鲀毒素的提取与检测[J].上海海洋大学学报,2017,26(6):801-807.
ZHANG Na,WEI Fen,BAO Baolong.Extraction and detection of tetrodotoxin in Takifugu niphobles and its symbiotic bacteria[J].Journal of Shanghai Ocean University,2017,26(6):801-807.
星点东方鲀及共生细菌河鲀毒素的提取与检测
Extraction and detection of tetrodotoxin in Takifugu niphobles and its symbiotic bacteria
投稿时间:2017-03-16  修订日期:2017-05-25
DOI:10.12024/jsou.20170301982
中文关键词: 星点东方鲀  河鲀毒素  液相色谱-串联质谱  共生细菌
英文关键词: Takifugu niphobles  Tetrodotoxin  LC-MS/MS  Symbiotic bacteria
基金项目:国家自然科学基金(41176108)
作者单位E-mail
张娜 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室, 上海 201306  
韦芬 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室, 上海 201306  
鲍宝龙 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室, 上海 201306 blbao@shou.edu.cn 
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中文摘要:
      从福建东山县附近海域捕获3尾野生星点东方鲀(Takifugu niphobles),采用液相色谱-串联质谱法检测了其组织及从组织分离到的细菌发酵产物的河鲀毒素(Tetrodotoxin,TTX)含量,结果显示星点东方鲀卵巢、肝脏和肌肉组织的TTX含量分别是56.247、0.380和0.118 μg/g。根据16S rDNA测序的结果,从星点东方鲀的肠道中分离到一株鳗利斯顿氏菌属(Listonella sp.)细菌,从肝脏中分离到两种不动杆菌属(Acinetobacter sp.)细菌,从肌肉中分离到一株肠杆菌属(Enterobacter sp.)细菌,从卵巢组织中分离到一株牡蛎弧菌(Vibrio crassostreae),其中,除牡蛎弧菌和一株不动杆菌属细菌外,其他细菌的发酵液提取物中均检测到TTX。这是首次在河鲀鱼类组织中分离到产TTX的鳗利斯顿氏菌属细菌和不动杆菌属细菌。
英文摘要:
      To detect the tetrodotoxin (TTX) of Takifugu niphobles and its symbiotic bacteria, three individuals of T. niphobles were collected from the sea near County Dongshan, Zhangzhou, Fujian Province, China. The collected fishes T. niphobles owned some typical morphological features, such as a lot of white dots spreading all over the dorsal body, each of oval black patches is at the base of dorsal fin or behind pectoral fin in each body side, each fins showing light yellow color. According to the test results of liquid chromatography coupled with mass spectrometry (LC-MS/MS), the amount of TTX in ovary, liver and muscle tissue contain 56.247, 0.380 and 0.118 μg/g respectively. Based on the 16S rDNA sequence identity and phylogenetic analysis with known bacterial species in GenBank database, one bacterial strain Listonella sp. from intestines, Enterobacter sp. from muscle and Vibrio crassostreae from ovary, two bacteria Acinetobacter sp. from liver, were isolated in this study. According to the LC-MS/MS result, three bacteria can produce TTX except Vibrio crassostreae and one bacterial strain Acinetobacter sp.. 1013 CFU of Acinetobacter lwoffii, Enterobacter sp. and Listonella sp. can produce 0.855, 0.318, and 0.298 ng TTX, respectively. This is the first report that the bacteria of Listonella and Acinetobacter isolated from puffer fish can produce TTX, our research further enriched the catalogue of TTX-producing bacteria.
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