文章摘要
袁新程,戴习林,朱其建,李玉峰,胡彦杰,周迅,丁福江.罗氏沼虾5个专门化品系选择系繁殖力的比较分析[J].上海海洋大学学报,2017,26(5):674-681.
YUAN Xincheng,DAI Xilin,ZHU Qijian,LI Yufeng,HU Yanjie,ZHOU Xun,DING Fujiang.Comparative analysis of fecundity of 5 specialized strains of shrimp Macrobrachium rosenbergii[J].Journal of Shanghai Ocean University,2017,26(5):674-681.
罗氏沼虾5个专门化品系选择系繁殖力的比较分析
Comparative analysis of fecundity of 5 specialized strains of shrimp Macrobrachium rosenbergii
投稿时间:2017-05-11  修订日期:2017-05-26
DOI:10.12024/jsou.20170502052
中文关键词: 罗氏沼虾  专门化品系  繁殖力  卵黄蛋白原  组织蛋白酶D
英文关键词: Macrobrachium rosenbergii  specialized strains  fecundity  vitellogenin  cathepsin D
基金项目:上海市现代农业产业技术体系建设项目(沪农科产字〔2016〕第5号)
作者单位E-mail
袁新程 上海海洋大学 农业部淡水水产种质资源重点实验室, 上海 201306  
戴习林 上海海洋大学 农业部淡水水产种质资源重点实验室, 上海 201306 xldai@shou.edu.cn 
朱其建 上海海洋大学 农业部淡水水产种质资源重点实验室, 上海 201306  
李玉峰 上海海洋大学 农业部淡水水产种质资源重点实验室, 上海 201306  
胡彦杰 上海海洋大学 农业部淡水水产种质资源重点实验室, 上海 201306  
周迅 上海海洋大学 农业部淡水水产种质资源重点实验室, 上海 201306  
丁福江 上海申漕特种水产开发公司, 上海 201516  
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中文摘要:
      为探讨罗氏沼虾繁殖性能,本实验通过测定5个罗氏沼虾专门化品系选择系(A、B、C、D、E 系)连续3次抱卵的单位体质量抱卵量(FW)、卵巢发育周期(CO)、受精卵卵径(DE)、卵黄蛋白原(VTG)含量、组织蛋白酶D(CTSD)活性等指标,比较分析5个选择系的繁殖力,以期筛选出高繁殖力品系罗氏沼虾。结果显示:罗氏沼虾绝对抱卵量(F)、FW与体质量正相关显著(P<0.05),F与FW呈极显著正相关(P<0.01);5个选择系的三次抱卵FW平均值范围为(1.96~2.30)×103粒/g,各系间差异显著(P<0.05),其中B和D的FW较高,分别为2.26×103粒/g和2.30×103粒/g;5个选择系受精卵中VTG含量、CTSD活性的平均值范围分别为3.48~5.80 μg/g、271.81~320.82 U/g,各系间差异均显著(P<0.05),其中受精卵VTG含量最高为B的5.80 μg/g,CTSD活性最高为D的320.82 U/g,次之为B的303.09 U/g,两者无差异(P>0.05);CO范围为18.8~23.8 d,第二次大于第一次(2~8 d),各系间无差异(P>0.05),但均显著小于对照组(P<0.05);DE平均值范围为542.3~552.0 nm,各系间无差异,B、D选择系DE均值较大,分别为551.7 nm、552.0 nm。因此可认为罗氏沼虾5个专门化品系选择系中B和D的繁殖力最强,A选择系次之,C和E选择系最弱,此可为罗氏沼虾专门化品系选择系的选育提供依据。
英文摘要:
      In order to investigate the breeding performance of Macrobrachium rosenbergii, this experiment was conducted by measuring 5 specialized strains of shrimp Macrobrachium rosenbergii (A, B, C, D, E) of the three consecutive spawning unit weight of fecundity (FW) ovarian development cycle (CO), fertilized egg diameter (DE), vitellogenin (VTG) content, and cathepsin D (CTSD) activity index, and comparative analysis of 5 selection strains of fertility was made, with a view to screening the high fecundity strains of Macrobrachium rosenbergii. The results showed that the absolute fecundity (F), FW and body weight were positively correlated (P<0.05), F and FW were also significantly positively correlated (P<0.01). The average value of FW of the continuous thrice spawning of the 5 selected strains was (1.96-2.30)×103 eggs/g, the differences between the strains were significant (P<0.05).The FW of B and D were 2.26×103 eggs/g and 2.30×103 eggs/g respectively. The average range of VTG content and CTSD activity in the 5 selected strains were 3.48-5.80 μg/g, 271.81-320.82 U/g, and there was significant difference among the strains (P<0.05),while the highest content of VTG in fertilized eggs was B (5.80 μg/g), the highest CTSD activity was D (320.82 U/g), the second was B (303.09 U/g), and there was no difference between them (P>0.05). The range of Co was 18.8-23.8 d, second time larger than the first (2-8 d), there was no difference (P>0.05) between each strain,which were all significantly lower than those in the control group (P<0.05). The mean value range of DE was 542.3-552.0 nm, there is no difference among strains, and the average egg diameters of B and D were larger, 551.7 nm and 552.0 nm, respectively. It can be confirmed that among 5 specialized strains of Macrobrachium rosenbergii, the fecundity of B and D is the strongest, A is weak, C and E is the weakest, which can provide basis for breeding of specialized strains of shrimp Macrobrachium rosenbergii.
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