To investigate the efficacy of cross-linked tilapia collagen chimeric notoginseng polysaccharide dressing （CLT-NPD） in promoting wound healing， the characteristics of wound healing in three types of CLT-NPD samples were compared. Quantitative notoginseng polysaccharides were uniformly distributed in the cross-linked tilapia collagen network to synthesize CLT-NPD. The crosslinking effect of CLT-NPD was analyzed by Fourier transform infrared （FT-IR） spectroscopy. Rats with injured skin were divided into blank group， positive control group and treatment group. The features of CLT-NPD in repairing wounds were detected from morphological indicators， immunological indicators and tissue biochemical indicators. The absorption peaks of amide A band， amide I band， and amide II band in the CLT-NPD samples were significantly enhanced. Compared with the blank group， the wound healing rate of the positive control group and the three treatment groups was significantly increased （P<0.05）， the regeneration of the epithelial cell layer was obvious， the content of type I collagen was significantly increased （P<0.05）， the levels of fibroblast growth factor （FGF） and epidermal growth factor （EGF） were significantly increased， and the levels of IL-1β， IL-6， IL-8 and TNF-α were significantly decreased （P<0.05）. Compared with the positive control group， there was no significant difference in the wound healing rate， type I collagen content， FGF and EGF levels， and IL-1β， IL-6， IL-8 and TNF-α levels among the three treatment groups， and the regeneration of the epithelial cell layer was similar. The levels of IL-1β， IL-8 and TNF-α in treatment group 3 on the third day were significantly lower than those in the other treatment groups， and the epithelial cell layer was smoother and more complete on the 14th day.CLT-NPD was successfully prepared. CLT-NPD achieved similar wound healing effects as those observed with the positive control dressing； notably Group 3 utilizing a 4∶1 ratio demonstrated optimal results.