To investigate the transcriptional regulatory mechanism of the slc5a1 gene promoter in the large yellow croaker （Larimichthys crocea）. The study employed bioinformatics methods to perform a phylogenetic tree analysis of the slc5a1 amino acid sequences from large yellow croaker and other species. Using large yellow croaker genomic DNA as a template， the slc5a1 gene promoter region was obtained by PCR amplification. Bioinformatics software was then utilized to predict and analyze the molecular structural features of this sequence， including the core promoter， regulatory elements， and transcription factors. Finally， the transcriptional activity of different fragments of the promoter region was assessed using a dual-luciferase reporter gene system. Bioinformatics analysis results showed that the slc5a1 amino acid sequence is highly conserved in fish species. Prediction of the large yellow croaker slc5a1 gene promoter region identified three potential core promoters， as well as binding sites for several transcription factors， including Sp1， C/EBPα， GATA-1， and Oct-1. The results from the dual-luciferase reporter gene system revealed that the region from -2 073 to -1 723 bp of the large yellow croaker slc5a1 gene promoter contains positive regulatory elements， the region from -1 723 to -1 404 bp contains positive regulatory elements， the region from -1 404 to -743 bp contains negative regulatory elements， the region from -743 to -272 bp contains negative regulatory elements， and the region from -272 to +153 bp contains no regulatory elements. The study indicates that the upstream region from -2 073 to -1 404 bp of the large yellow croaker slc5a1 gene is the core promoter of this gene. The research results lay the foundation for further investigation into the transcriptional regulatory mechanism of the large yellow croaker slc5a1 gene.