In order to prevent substandard or adulterated behaviour and to safeguard the authenticity of product labelling， authentication of marine fish-derived ingredients in surimi products is required. In this study， eight marine fish-specific primers/probes were designed based on the mitochondrial 16S ribosomal RNA （16S rRNA） gene to establish a real-time PCR （qPCR） method for the qualitative detection of marine fish-derived components in surimi products， and to analyse the detection limit， amplification efficiency and sensitivity of the method. Fifty samples of commercially available surimi were tested to verify the practical application of the method. The results showed that the method was highly specific and could specifically amplify the template DNA of eight species of marine fish. This method can detect DNA concentrations of 0.05 ng/μL for bluefin gurnard （Chelidonichthys kumu） and 0.5 ng/μL for red seabream （Pagrus major）， walleye pollock （Gadus chalcogrammus）， Atlantic cod （Gadus morhua）， blue whiting （Micromesistius poutassou）， saithe （Pollachius virens）， silver croaker （Pennahia argentata） and bay sillago （Sillago ingenuua）. The sensitivities were 0.1% for blue whiting， 0.5% for silver croaker， bluefin gurnard and saithe， 1% for atlantic cod， 5% for walleye pollock and bay sillago， and 10% for red seabream.Tests on 50 commercially available surimi samples revealed that one or more of the 8 components of Marine fish were detected in 29 samples. This result was consistent with that obtained using DNA barcoding， indicating that this method is accurate and reliable. This study can provide an effective method for the authenticity testing and traceability analysis of surimi and its products， and aims to provide technical support for the market supervision and quality control of raw materials in enterprises.