In order to study the vibrio anguillarum minus toadstool inactivated vaccine immune effect， this study attenuated strains of V. anguillarum Δvah1-vah4-rtxA for high temperature inactivated bacteria fermentation preparation into inactivated vaccines， 2% according to the proportion of added to the feed， oral immune Lateolabrax maculatus 21 d. Blood was collected from the caudal vertebral vein at 0 d， 7 d， 14 d and 21 d after immunization， and the serum levels of immunoglobulin M （IgM） and lysozyme （LZM） were measured. After 21 days of immunization， the fish were challenged with wild type V. anguillarum via intraperitoneal injection.The results showed that compared with the control group， IgM content in the serum of the immunized group of L. maculatus increased significantly and reached the highest value by the 21st day. Serum LZM content increased to reach the highest value by the 14th d， and thereafter， decreased slightly. Transcriptome analysis of the head kidney of L. maculatus for 21 d of immunization showed 252 significantly up-regulated genes and 200 significantly down-regulated genes compared to the control group， and KEGG analysis showed that these differential genes were significantly enriched for hematopoietic cell spectrum， natural killer cell-mediated cytotoxicity， phagolysosomes， the NF -κB signaling pathway， the production of IgA by intestinal immune network， and the Fc epsilon RI and other pathways. The relative immunoprotection rates at 7 d and 14 d were 62.5% and 25.0%， respectively. Pathological observations of the immunized group showed that the extent of vacuolization of the intestinal mucosa and structural damage to the gill tissues were relatively mild.The above results indicated that the attenuated strain of V. anguillarum Δvah1-vah4-rtxA preparation into inactivated vaccine， to improve the flower L. maculatus against V. anguillarum infection have better effect.