This study was conducted to investigate the effects of different gradients of Ala-Gln added to fishmeal-free diets on the growth performance， amino acid metabolism and energy budget of juvenile Acipenser schrenckii. In this study， 450 juvenile sturgeon with initial body weight of （21.66±1.02） g were raised in a recirculating water culture facility and randomly divided into 5 groups （3 replicates per group with 30 fish per replicate）. The control group was fed a basal diet， while the experimental groups were fed diets supplemented with 0， 0.25%， 0.50%， 0.75%， and 1.00% Ala-Gln， respectively. The experiment lasted for 56 days. The results were as follows： （1） The final weight and condition factor of sturgeon were significantly higher in the groups receiving 0.50%-1.00% Ala-Gln compared to the control group （P<0.05）. Weight gain rate， feed efficiency， dry matter intake， crude protein， and crude lipid content increased with Ala-Gln supplementation， though these increases did not reach statistical significance （P>0.05）. （2） Fecal energy （F）， excretion energy （U）， showed no significant differences （P>0.05）， but growth energy （G） and metabolizable energy （R） were significantly different from the control group （P<0.05）. （3） The cysteine content of fish supplemented with 0.75%Ala-Gln was significantly higher than that of other groups （P<0.05）. The glycine content of fish supplemented with 0.50%Ala-Gln was significantly higher than that of other groups （P<0.05）. The glycine content of fish supplemented with 0.50%Ala-Gln was significantly higher than that of other groups （P<0.05）. （4） Glutamine and alanine levels in the blood were significantly increased 5 hours after ingestion （P<0.05）. After 0.25%-1.00% Ala-Gln supplementation， glutamine was the most important amino acid affecting the blood metabolic profile. The addition of 0.50% Ala-Gln resulted in significantly higher aspartic acid levels compared to other groups （P<0.05）. Analysis of PLA， a key component of aspartic acid， indicated that glutamine was the most important amino acid influencing the blood metabolic profile. The results indicate that exogenous supplementation of 0.75% to 1.00% Ala-Gln can significantly improve the growth performance of juvenile sturgeon， likely due to increased absorption of Ala-Gln， which elevates the levels of glutamine and alanine in the body， thereby affecting metabolism and promoting protein synthesis.