In order to investigate the response characteristics of the contact behavior of Chinese sturgeon （Acipenser sinensis） in the ovary during the early stage of reproduction to flow velocity in natural water bodies， this study set up ovaries in the Yangtze River estuary in November 2024. The flow velocity was regulated by a push flow pump to form different flow velocity environments， and the intensity of the contact behavior of Chinese sturgeon under different flow velocity conditions was statistically analyzed. The results showed that： （1） Under the condition of moderate flow velocity （0.12-0.20 m/s）， the intensity of contact behavior of Chinese sturgeon was significantly higher than that of natural flow velocity （0.05-0.15 m/s） （P<0.01）， and the increase in contact behavior intensity of Chinese sturgeon in each region reached more than 50%. Among them， the contact behavior intensity in the low and medium flow velocity region B and the high flow velocity region A was the highest， reaching （50.10±2.93）times/h and （47.80±5.61）times/h， respectively. However， when the flow velocity was too high （>1.0 m/s）， the contact behavior intensity of Chinese sturgeon decreased slightly， with the highest decrease of 28.0% in the high flow velocity region. （2） The proportion of contact behavior in the low to medium flow velocity zone B is always the highest， gradually increasing from 29.1% at the initial flow velocity to 35.0% with the increase of flow velocity， and significantly higher than other zones under high flow velocity conditions （P<0.01）. Research has shown that flow velocity has a nonlinear regulatory effect on the contact behavior of Chinese sturgeons. Moderately increasing flow velocity can maximize the stimulation of their contact behavior. At the same time， during the early stages of reproduction， Chinese sturgeons have the ability to autonomously choose water flow， and their contact behavior is more inclined to occur in areas with low to medium flow velocities，This study provides a key theoretical basis for optimizing the velocity regulation of the spawning environment of Acipenser sinensis and repairing the habitat of the natural spawning ground.