Glyptosternum maculatum is an important cold-water economic fish in Tibet. To explore the differences in nutritional value of wild and farmed Glyptosternum maculatum muscles， the conventional nutrients， amino acids， and fatty acids in the muscles of wild， domesticated， and farmed Glyptosternum maculatum were determined. The results showed that there were significant differences between the conventional nutritional components of wild， domesticated and farmed Glyptosternum maculatum （P<0.05）； the crude fat mass score of wild fish was significantly higher than that of domesticated fish and farmed fish （P<0.05）； the crude protein mass score of domesticated fish was considerably higher than that of wild fish and farmed fish （P<0.05）； the moisture mass score of farmed fish was significantly higher than that of wild fish and farmed fish （P<0.05）. 17 amino acids were detected in wild， domesticated， and farmed Glyptosternum maculatum， among which there were significant differences in total amino acid content between farmed fish and domesticated fish （P<0.05）. The highest farmed fish were followed by wild fish and domesticated fish， and the lowest farmed fish， and the total non-essential amino acid content of wild fish， domesticated fish， and farmed fish was consistent with the total amino acid content. According to the AAS and CS standards， the first restriction amino acids of wild， domesticated， and farmed Glyptosternum maculatum are methionine + cystine， and the second restriction amino acid is valine. The total amount of saturated fatty acids， monounsaturated fatty acids， and polyunsaturated fatty acids content in wild fish was significantly higher than that in domesticated fish and farmed fish （P<0.05）. Among them， C12：0， C17：0， and C18：3n6w are fatty acids unique to wild fish， C22：1n9， C24：1n9， and C22：2n6 are fatty acids unique to farmed fish， and C15：0 and C20：0 are unique to wild fish and farmed fish. Both wild fish and farmed fish DHA+EPA content was significantly higher than domesticated fish （P<0.05）. In summary， the nutritional quality of wild and farmed Glyptosternum maculatum is higher than that of domesticated Glyptosternum maculatum， which provides basic data for the improvement of Glyptosternum maculatum muscle quality.